Research Article

“In-gel patch electrophoresis:” A new method for environmental DNA purification

  1. Changhyun Roh1,2,
  2. Francois Villatte2,
  3. Byung-Gee Kim1,
  4. Rolf D. Schmid Prof.2,*

Article first published online: 22 JUL 2005

DOI: 10.1002/elps.200410328

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 26, Issue 16, pages 3055–3061, No. 16 August 2005

Additional Information

How to Cite

Roh, C., Villatte, F., Kim, B.-G. and Schmid, R. (2005), “In-gel patch electrophoresis:” A new method for environmental DNA purification. ELECTROPHORESIS, 26: 3055–3061. doi: 10.1002/elps.200410328

Author Information

  1. 1

    School of Chemical Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea

  2. 2

    Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany

*Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany Fax: +49-711-685-3196

Publication History

  1. Issue published online: 16 AUG 2005
  2. Article first published online: 22 JUL 2005
  3. Manuscript Received: 16 NOV 2004

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Keywords:

  • In-gel patch electrophoresis;
  • Metagenome;
  • Purification

Abstract

Most of the microorganism species are largely untapped and could represent an interesting reservoir of genes useful for biotechnological applications. Unfortunately, a major difficulty associated with the methods used to isolate environmental DNA is related to the contamination of the extracted material with humic substances. These polyphenolic compounds inhibit the DNA processing reactions and severely impede cloning procedures. In this work, we describe a rapid, simple, and efficient method for the purification of genomic DNA from environmental samples: we added a chromatography step directly embedded into an agarose gel electrophoresis. This strategy enabled the DNA extraction from various environmental samples and it appeared that the purity grade was compatible with digestion by restriction enzymes and polymerase chain reaction (PCR) amplifications.

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