Research Article

Introduction of an single nucleodite polymorphism-based “Major Y-chromosome haplogroup typing kit” suitable for predicting the geographical origin of male lineages

  1. María Brión Dr.1,*,
  2. Juan J. Sanchez2,
  3. Kinga Balogh3,
  4. Catherine Thacker4,
  5. Alejandro Blanco-Verea1,
  6. Claus Børsting2,
  7. Beate Stradmann-Bellinghausen3,
  8. Magdalena Bogus3,
  9. Denise Syndercombe-Court4,
  10. Peter M. Schneider3,
  11. Angel Carracedo1,
  12. Niels Morling2

Article first published online: 7 NOV 2005

DOI: 10.1002/elps.200500293

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 26, Issue 23, pages 4411–4420, No. 23 December 2005

Additional Information

How to Cite

Brión, M., Sanchez, J., Balogh, K., Thacker, C., Blanco-Verea, A., Børsting, C., Stradmann-Bellinghausen, B., Bogus, M., Syndercombe-Court, D., Schneider, P., Carracedo, A. and Morling, N. (2005), Introduction of an single nucleodite polymorphism-based “Major Y-chromosome haplogroup typing kit” suitable for predicting the geographical origin of male lineages. ELECTROPHORESIS, 26: 4411–4420. doi: 10.1002/elps.200500293

Author Information

  1. 1

    Institute of Legal Medicine, National Genotyping Center (CEGEN), University of Santiago de Compostela, Spain

  2. 2

    Department of Forensic Genetics, Institute of Forensic Medicine, University of Copenhagen, Denmark

  3. 3

    Institute of Legal Medicine, University of Mainz, Germany

  4. 4

    Barts and the London, Queen Mary's School of Medicine and Dentistry, London, UK

*Institute of Legal Medicine, National Genotyping Center (CEGEN), University of Santiago de Compostela, San Francisco s/n. E-15782, Santiago de Compostela, Spain Fax: +34-98-158-0336

Publication History

  1. Issue published online: 28 NOV 2005
  2. Article first published online: 7 NOV 2005
  3. Manuscript Received: 15 APR 2005

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Keywords:

  • Capillary electrophoresis;
  • Geographic origin;
  • Multiplex;
  • Single base extension;
  • Y-SNPs

Abstract

The European Consortium “High-throughput analysis of single nucleotide polymorphisms for the forensic identification of persons – SNPforID”, has performed a selection of candidate Y-chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y-Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A “Major Y-chromosome haplogroup typing kit” has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y-chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage.

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