Research Article

Micellar electrokinetic capillary chromatographic method for the quantitative analysis of uricosuric and antigout drugs in pharmaceutical preparations

  1. Hwang-Shang Kou,
  2. Tsai-Pei Lin,
  3. Tang-Chia Chung,
  4. Hsin-Lung Wu Professor*

Article first published online: 22 MAY 2006

DOI: 10.1002/elps.200500860

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Special Issue: PHARMACEUTICAL ANALYSIS

Volume 27, Issue 12, pages 2293–2299, No. 12 June 2006

Additional Information

How to Cite

Kou, H.-S., Lin, T.-P., Chung, T.-C. and Wu, H.-L. (2006), Micellar electrokinetic capillary chromatographic method for the quantitative analysis of uricosuric and antigout drugs in pharmaceutical preparations. ELECTROPHORESIS, 27: 2293–2299. doi: 10.1002/elps.200500860

Author Information

  1. Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan

*Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan Fax: +886-7-3159597

Publication History

  1. Issue published online: 19 JUN 2006
  2. Article first published online: 22 MAY 2006
  3. Manuscript Received: 22 NOV 2005

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Keywords:

  • MEKC-UV;
  • Pharmaceutical analysis;
  • Uricosuric and antigout drugs

Abstract

A simple MEKC method is described for the separation and quantification of seven widely used uricosuric and antigout drugs, including allopurinol (AP), benzbromarone (BZB), colchicine (COL), orotic acid (OA), oxypurinol (OP), probenecid (PB), and sulfinpyrazone (SPZ). The drugs were separated in a BGE of borate buffer (45 mM; pH 9.00) with SDS (20 mM) as the micellar source and the separated drugs were directly monitored with a UV detector (214 nm). Several parameters affecting the separation and analysis of the drugs were studied. Based on the normalized peak-area ratios of the drugs to an internal standard versus the concentration of the drugs, the method is applicable to quantify BZB, COL, and SPZ (each 5–200 μM), AP, OA, OP, and PB (each 10–200 μM) with detection limits (S/N = 3, 0.5 psi, 5 s injection) in the range of 0.6–4.0 μM. The precision (RSD; n = 5) and accuracy (relative error; n = 5) of the method for intraday and interday analyses of the analytes at three levels (30, 120, and 180 μM) are below 4% (n = 3). The method was demonstrated to be suitable for the analysis of AP and COL in commercial tablets with speed and simplicity.

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