Microfluidic cell disruption system employing a magnetically actuated diaphragm

Authors

  • Yun Suk Huh,

    1. Separation Process Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
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    • These authors contributed equally.

  • Jong Hyun Choi,

    1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Ultramicrochemical Process Systems, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon, Korea
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    • These authors contributed equally.

  • Tae Jung Park,

    1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Ultramicrochemical Process Systems, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon, Korea
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  • Yeon Ki Hong,

    1. Department of Chemical and Biological Engineering, Chungju National University, Chungju, Chungbuk, Korea
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  • Won Hi Hong Professor,

    1. Separation Process Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
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    • Additional corresponding author

  • Sang Yup Lee Professor

    Corresponding author
    1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Ultramicrochemical Process Systems, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon, Korea
    2. Department of Bio and Brain Engineering and Bioinformatics Research Center, KAIST, Daejeon, Korea
    • Correspondences: Professor Sang Yup Lee, Department of Chemical and Biomolecular Engineering, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea Fax: +82-42-869-3910
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Errata

This article is corrected by:

  1. Errata: Microfluidic cell disruption system employing a magnetically actuated diaphragm Volume 29, Issue 13, 2927, Article first published online: 10 July 2008
  2. Errata: Microfluidic cell disruption system employing a magnetically actuated diaphragm Volume 29, Issue 9, 1954, Article first published online: 28 April 2008

Abstract

A microfluidic cell lysis chip equipped with a micromixer and SPE unit was developed and used for quantitative analysis of intracellular proteins. This miniaturized sample preparation system can be employed for any purpose where cell disruption is needed to obtain intracellular constituents for the subsequent analysis. This system comprises a magnetically actuated micromixer to disrupt cells, a hydrophobic valve to manipulate the cell lysate, and a packed porous polymerized monolith chamber for SPE and filtering debris from the cell lysate. Using recombinant Escherichia coli expressing intracellular enhanced green fluorescent protein (EGFP) and lipase as model bacteria, we optimized the cell disruption condition with respect to the lysis buffer composition, mixing time, and the frequency of the diaphragm in the micromixer, which was magnetically actuated by an external magnetic stirrer in the micromixer chamber. The lysed sample prepared under the optimal condition was purified by the packed SPE in the microfluidic chip. At a frequency of 1.96 Hz, the final cell lysis efficiency and relative fluorescence intensity of EGFP after the cell disruption process were greater than 90 and 94%, respectively. Thus, this microfluidic cell disruption chip can be used for the efficient lysis of cells for further analysis of intracellular contents in many applications.

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