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Comparison of the analytical performance of electrophoresis microchannels fabricated in PDMS, glass, and polyester-toner

Authors

  • Wendell Karlos Tomazelli Coltro,

    1. Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil
    2. Ralph N. Adams Institute for Bioanalytical Chemistry, The University of Kansas, Lawrence, Kansas, USA
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  • Susan M. Lunte,

    1. Ralph N. Adams Institute for Bioanalytical Chemistry, The University of Kansas, Lawrence, Kansas, USA
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  • Emanuel Carrilho

    Corresponding author
    1. Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil
    • Instituto de Química de São Carlos, Universidade de São Paulo, Grupo de Bioanalítica, Microfabricação e Separações, Avenida Trabalhador São-carlense 400, P.O. Box 780, 13560-970 São Carlos, SP, Brazil Fax: +55-16-3373-9985
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Abstract

This paper compares the analytical performance of microchannels fabricated in PDMS, glass, and polyester-toner for electrophoretic separations. Glass and PDMS chips were fabricated using well-established photolithographic and replica-molding procedures, respectively. PDMS channels were sealed against three different types of materials: native PDMS, plasma-oxidized PDMS, and glass. Polyester-toner chips were micromachined by a direct-printing process using an office laser printer. All microchannels were fabricated with similar dimensions according to the limitations of the direct-printing process (width/depth 150 μm/12 μm). LIF was employed for detection to rule out any losses in separation efficiency due to the detector configuration. Two fluorescent dyes, coumarin and fluorescein, were used as model analytes. Devices were evaluated for the following parameters related to electrophoretic separations: EOF, heat dissipation, injection reproducibility, separation efficiency, and adsorption to channel wall.

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