Nucleic Acids

Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning-based approach

  1. Matthew J. Nolan1,
  2. Aaron R. Jex1,
  3. Jacqui A. Upcroft2,
  4. Peter Upcroft2,
  5. Robin B. Gasser1,*

Article first published online: 17 AUG 2011

DOI: 10.1002/elps.201100283

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 32, Issue 16, pages 2075–2090, August 2011

Additional Information

How to Cite

Nolan, M. J., Jex, A. R., Upcroft, J. A., Upcroft, P. and Gasser, R. B. (2011), Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning-based approach. ELECTROPHORESIS, 32: 2075–2090. doi: 10.1002/elps.201100283

Author Information

  1. 1

    Department of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia

  2. 2

    Queensland Institute of Medical Research, Brisbane, Queensland, Australia

*Department of Veterinary Science, The University of Melbourne, Parkville, Victoria 3052, Australia Fax: +61-3-97312366

Publication History

  1. Issue published online: 17 AUG 2011
  2. Article first published online: 17 AUG 2011
  3. Manuscript Revised: 10 JUN 2011
  4. Manuscript Accepted: 10 JUN 2011
  5. Manuscript Received: 24 MAY 2011

Funded by

  • Australian Research Council, Melbourne Water Corporation
  • The Australian Academy of Science and Fulbright Commission
  • National Institutes of Health, USA. Grant Number: U01 Cooperative Research Agreement AI75527
  • National Health and Medical Research Council of Australia. Grant Number: 496640

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Keywords:

  • Barcoding;
  • Giardia duodenalis;
  • SSCP;
  • Restriction endonuclease fingerprinting

Abstract

We barcoded 25 in vitro isolates (representing 92 samples) of Giardia duodenalis from humans and other animals, which have been assembled by the Upcroft team at the Queensland Institute of Medical Research over a period of almost three decades. We used mutation scanning-coupled sequencing of loci in the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes, combined with phylogenetic analysis, to genetically characterise them. Specifically, the isolates (n=14) of G. duodenalis from humans from Australia (AD113; BRIS/83/HEPU/106; BRIS/87/HEPU/713; BRIS/89/HEPU/1003; BRIS/92/HEPU/1541; BRIS/92/HEPU/1590; BRIS/92/HEPU/2443; BRIS/93/HEPU/1706), Malaysia (KL/92/IMR/1106) and Afghanistan (WB), a cat from Australia (BAC2), a sheep from Canada (OAS1) and a sulphur-crested cockatoo from Australia (BRIS/95/HEPU/2041) represented assemblage A (sub-assemblage AI-1, AI-2 or AII-2); isolates (n=10) from humans from Australia (BRIS/91/HEPU/1279; BRIS/92/HEPU/2342; BRIS/92/HEPU/2348; BRIS/93/HEPU/1638; BRIS/93/HEPU/1653; BRIS/93/HEPU/1705; BRIS/93/HEPU/1718; BRIS/93/HEPU/1727), Papua New Guinea (BRIS/92/HEPU/1487) and Canada (H7) represented assemblage B (sub-assemblage BIV) and an isolate from cattle from Australia (BRIS/92/HEPU/1709) had a match to assemblage E. Isolate BRIS/90/HEPU/1229 from a human from Australia was shown to represent a mixed population of assemblages A and B. These barcoded isolates (including stocks and derived lines) now allow direct comparisons of experimental data among laboratories and represent a massive resource for transcriptomic, proteomic, metabolic and functional genomic studies using advanced molecular technologies. (Nucleotide sequences reported in this paper are available in the GenBank database under accession nos. JN204442–JN204461.)

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