Microfluidics and Miniaturization

Poly(ethylene glycol)-coated microfluidic devices for chip electrophoresis

  1. Marcel Schulze,
  2. Detlev Belder*

Article first published online: 5 JAN 2012

DOI: 10.1002/elps.201100401

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 33, Issue 2, pages 370–378, January 2012

Additional Information

How to Cite

Schulze, M. and Belder, D. (2012), Poly(ethylene glycol)-coated microfluidic devices for chip electrophoresis. ELECTROPHORESIS, 33: 370–378. doi: 10.1002/elps.201100401

Author Information

  1. University of Leipzig, Institute of Analytical Chemistry, Leipzig, Germany

*Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, D-04103 Leipzig, Germany Fax: +49-341-9736115

Publication History

  1. Issue published online: 5 JAN 2012
  2. Article first published online: 5 JAN 2012
  3. Manuscript Revised: 1 SEP 2011
  4. Manuscript Accepted: 1 SEP 2011
  5. Manuscript Received: 22 JUL 2011

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Keywords:

  • Hydrophilic coating;
  • Microchip;
  • Microchip electrophoresis;
  • Surface modification

Abstract

Herein, we report on a strategy for durable modification of the channel surface in microfluidic glass chips with the neutral hydrophilic-coating material poly(ethylene glycol) PEG-1M-100. Applied in microchip electrophoresis such PEG-coated devices exhibit a suppressed electroosmotic flow and reduced analyte adsorption. The PEG-coated chips were successfully applied in chip electrophoresis of FITC-labelled amines and amino acids and native proteins as well as in chiral separations. The performance of the coated chips was found to be superior compared with uncoated microchips. The coated chips exhibited high stability and the relative standard deviation of migration times in PEG-coated devices was less than 2%.

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