Research Article

Rapid and sensitive analysis of three polyphenols in tobacco by CE using homemade C4D with a mini detection cell

  1. Fuwei Xie1,2,
  2. Yanhao Zhang1,
  3. Bo Zheng3,
  4. Feifei Xu1,
  5. Jianpo Su4,
  6. Yanyan Lu1,
  7. Fanya Zeng4,
  8. Bin Zhang4,*,
  9. Yaxiao Guo1,
  10. Shusheng Zhang1,*

Article first published online: 9 AUG 2012

DOI: 10.1002/elps.201200057

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Special Issue: Advanced Food Analysis and Foodomics

Volume 33, Issue 15, pages 2433–2440, August 2012

Additional Information

How to Cite

Xie, F., Zhang, Y., Zheng, B., Xu, F., Su, J., Lu, Y., Zeng, F., Zhang, B., Guo, Y. and Zhang, S. (2012), Rapid and sensitive analysis of three polyphenols in tobacco by CE using homemade C4D with a mini detection cell. ELECTROPHORESIS, 33: 2433–2440. doi: 10.1002/elps.201200057

Author Information

  1. 1

    College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, P. R. China

  2. 2

    Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, P. R.China

  3. 3

    Machinery College, Zhengzhou University, Zhengzhou, P. R. China

  4. 4

    Physical Engineering College, Zhengzhou University, Zhengzhou, P. R. China

*Correspondence: Dr. Shusheng Zhang, Department of Chemistry, College of Chemistry and Molecular Engineering, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, P. R. China
E-mail: zhangshusheng@371.net
Fax: +86-371-67766667
Additional corresponding author: Dr. Bin Zhang, E-mail: zhangbin@zzu.edu.cn

  1. Colour Online: See the article online to view Fig. 3 and 4 in colour.

Publication History

  1. Issue published online: 9 AUG 2012
  2. Article first published online: 9 AUG 2012
  3. Accepted manuscript online: 12 JUN 2012 12:49AM EST
  4. Manuscript Accepted: 17 APR 2012
  5. Manuscript Revised: 11 APR 2012
  6. Manuscript Received: 19 OCT 2011

Funded by

  • NSF of China. Grant Numbers: 20875083, 21077095
  • Innovation Scientists & Technicians Troop Construction Projects of Zhengzhou City. Grant Number: 10LJRC192

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Keywords:

  • Capillary electrophoresis;
  • C4D detection;
  • Polyphenols;
  • Tobacco

A rapid, sensitive, and practical CE with C4D detection was developed for the analysis of three polyphenols (rutin, scopoletin, and chlorogenic acid) in tobacco samples. The constructed mini detection cell (12 mm × 10 mm × 10 mm) of C4D featured with small inner cell volume (∼2 nL), smaller noise (<0.9 mV), repeatability, high strength and durableness. Three polyphenols were ultrasonically extracted with methanol–water (70:30, v/v) solution following SPE cleanup. The CE method was optimized with the running buffer of 150 mmol L−1 2-amino-2-methyl-1-propanol (pH 11.2), and the applied separation voltage of +20 kV over a capillary of 50 μm id × 375 μm od × 50 cm (38 cm to the C4D window, 41.5 cm to the UV detector window), which gave a baseline separation of three polyphenols within ca. 6 min. The method provided the limits of quantification (S/N = 10) at about 0.08–0.15 μg g−1 for three polyphenols, whereas the overall recoveries ranged from 82% to 88%. The proposed method has been successfully applied to measure three polyphenols in the actual tobacco samples, and their contents were calculated and evaluated.

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