Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation


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Correspondence: Dr. Michael Quail, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK

E-mail: mq1@sanger.ac.uk

Fax: +44-1223-494819


Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries.