Colour Online: See the article online to view Figs. 1–4 in colour.
Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation
Article first published online: 12 NOV 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Next Generation Sequencing and Genotyping
Volume 33, Issue 23, pages 3521–3528, December 2012
How to Cite
Quail, M. A., Gu, Y., Swerdlow, H. and Mayho, M. (2012), Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation. ELECTROPHORESIS, 33: 3521–3528. doi: 10.1002/elps.201200128
- Issue published online: 4 DEC 2012
- Article first published online: 12 NOV 2012
- Accepted manuscript online: 7 SEP 2012 06:11AM EST
- Manuscript Accepted: 12 MAY 2012
- Manuscript Revised: 25 APR 2012
- Manuscript Received: 27 FEB 2012
- Wellcome Trust. Grant Number: 098051
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|elps4435-sup-0001-FigureS1.ppt||402K||Supporting Information Figure 1. Reproducibility of size selection using Caliper Labchip XT, Pippin Prep and gel size selection. Agilent Bioanalyser electropherograms of 12 libraries independently size selected using; (A) LabChip XT was set to 700±12%; (B) Pippin Prep using 1.5% cassette collecting 500–700bp and (C) agarose gel with excision between 550 and 750bp.|
|elps4435-sup-0002-FigureS2.ppt||321K||Supporting Information Figure 2. Sequence representation versus GC content for 15x depth randomly normalized sequence coverage from Illumina libraries size selected using gel, AMPure XP, Caliper Labchip XT or Pippin Prep on: (A) Bordetella pertussis; (B) S. Pullorum, (C) S. aureus and (D) P. falciparum genomes.|
|elps4435-sup-0003-S1.doc||28K||Supporting Information S1|
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