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S1. FCM calculation and physical simulations on COMSOL Multiphysics – conditions and approximations used

S2. Data acquisition and treatment

Figure S1. Image treatment of the frames extracted from videos.

Figure S2. Theelectrical conductivity of LB and TSB media used for cell suspensions.

Figure S3. DEP force simulation with no flow (A) and (C) are simulations of the dielectrophoretic force generated within the quadrupole region next to the electrode plane for of 13 × 105 V/m and FCM values of 0.6 and −0.3, respectively.

Figure S4. Effect of flow on nDEP and pDEP trapping of E. coli cells in a microfluidic channel.

Figure S5. Dependence of the calculated DEP force for nDEP (A) and pDEP (B) with applied voltage, Vpp, at different heights in the microchannel above the electrode plane.

Figure S6. Comparison between DEP force and fluid drag force for nDEP (A) and pDEP (B).

Table S1. E. coli dielectric parameters used in the multishell model.

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