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Figure S1. Impedance magnitude (a) and phase (b) of the DEP device filled with buffers at medium conductivities of 5×10−4 S/m (+), 0.034 S/m (*, separation point 1), 0.058 S/m (◊, separation point 2), and 0.18 S/m (○, separation point 3).

Figure S2. Percentile voltage drop on electrodes at medium conductivities of 5×10−4 S/m (+), 0.034 S/m (*, separation point 1), 0.058 S/m (◊, separation point 2), and 0.18 S/m (○, separation point 3).

Figure S3. Percentile voltage drop across cell suspension at medium conductivities of 5×10−4 S/m (+), 0.034 S/m (*, separation point 1), 0.058 S/m (◊, separation point 2), and 0.18 S/m (○, separation point 3).

Figure S4. Original (black lines) and normalized (gray lines) separability parameter for buffer conductivities 0.034 S/m (solid line), 0.058 S/m (dashed line), and 0.18 S/m (dotted line).

Table S1. Geometrical and dielectric properties of Jurkat and B16 cells determined from the microfluidic impedance spectroscopy.

Table S2. Specific Double Layer Capacitance.

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