Development and optimization of an integrated PDMS based-microdialysis microchip electrophoresis device with on-chip derivatization for continuous monitoring of primary amines

Authors

  • Pradyot Nandi,

    1. Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA
    2. Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA
    Current affiliation:
    1. Center for Pharmaceutical Biotechnology, Department of Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO, USA
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  • David E. Scott,

    1. Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA
    2. Department of Chemistry, University of Kansas, Lawrence, KS, USA
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  • Dhara Desai,

    1. Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA
    2. Department of Chemistry, University of Kansas, Lawrence, KS, USA
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  • Susan M. Lunte

    Corresponding author
    1. Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA
    2. Department of Chemistry, University of Kansas, Lawrence, KS, USA
    • Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA
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  • Colour Online: See the article online to view Figs. , in colour.

Correspondence: Professor Susan M. Lunte, Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, KS 66047, USA

E-mail: slunte@ku.edu

Fax: +1-785-864-1916

Abstract

An all-PDMS on-line microdialysis-microchip electrophoresis with on-chip derivatization and electrophoretic separation for near real-time monitoring of primary amine-containing analytes is described. Each part of the chip was optimized separately, and the effect of each of the components on temporal resolution, lag time, and separation efficiency of the device was determined. Aspartate and glutamate were employed as test analytes. Derivatization was accomplished with naphthalene-2,3,-dicarboxyaldehyde/cyanide (NDA/CN), and the separation was performed using a 15-cm serpentine channel. The analytes were detected using LIF detection.

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