Current address: Departments of Biomedical Engineering and Chemistry, University of North Carolina, Chapel Hill, NC, USA.
Simultaneous detection of 19 K-ras mutations by free-solution conjugate electrophoresis of ligase detection reaction products on glass microchips
Article first published online: 24 JAN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 34, Issue 4, pages 590–597, February 2013
How to Cite
Albrecht, J. C., Kotani, A., Lin, J. S., Soper, S. A. and Barron, A. E. (2013), Simultaneous detection of 19 K-ras mutations by free-solution conjugate electrophoresis of ligase detection reaction products on glass microchips. ELECTROPHORESIS, 34: 590–597. doi: 10.1002/elps.201200462
Colour Online: See the article online to view Fig. 3 in colour.
- Issue published online: 18 FEB 2013
- Article first published online: 24 JAN 2013
- Accepted manuscript online: 28 NOV 2012 07:52PM EST
- Manuscript Accepted: 23 OCT 2012
- Manuscript Revised: 19 OCT 2012
- Manuscript Received: 21 AUG 2012
- National Institutes of Health. Grant Numbers: 5R01HG002918–04, 5R01HG001970-09, R21-1CA128671, 1RC2HG005596–01
- National Science Foundation
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Table S1. Sequence of LDR templates with each potential mutation in codons 12, 13, and 61 of the K-ras gene.
Table S2. Sequence of LDR discriminating and common primers, total LDR product length, and primer – drag-tag pairings.
Figure S1. Schematic illustrating how LDR-FSCE works.
Figure S2. Individual capillary electropherograms of LDR-FSCE products.
Figure S3. CE separations of all 19 LDR-FSCE products at varied electric field strengths.
Figure S4. Individual LDR-FSCE separation on a glass microchip.
Figure S5. Microchip separation of all 19 LDR-FSCE products at E = 700 V/cm.
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