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Figure S1. Counting of the Bacteriophage T4r by double layer agar (DLA) method. High titer of the phages was serially diluted with nutrient peptone broth by tenfold at each step. DLA plates was prepared by mixing the 100 μl of overnight culture of bacterial host (E. coli B) and 1 mL diluted phage in molten soft agar and pouring the mixture on top of the base layer agar. Plates were incubated overnight at 37 °C till the plaques were formed. The infective titers of the phages were calculated by the number of plaques formed on the assay plates. Assay dilutions of 10−5 to 10−9 were sufficient to determine the titer. Purple-pink color was due to gram staining of E. coli B. The transparent spots are plaques formed on the base layer agar. The number of plaque on the plate which could be clearly counted with reliable statistics was used for the concentration calculation.

Figure S2. Transmission Electron Microscope Images of the two types of bacteriophages used in this study.

Figure S3. The excitation and fluorescence emission spectra of Bacteriophage T4r labeled with SYGR green –I.

Table 1. Correlation of the input volumetric flow rate (in μl·min-1) and the observed linear flow velocity above the active area of 200 μm × 200 μm nanoelectrode array for the experiments with Bacteriophage T4r. The linear flow velocity was calculated by measuring the length of the stretched lines as virus particles flowing within the exposure time (varying between 0.2 to 0.5 s) of the video frames.

Table 2. Calculation of the efficiency of DEP capture with the low-concentration Bacteriophage T4r at 10 Hz and 10 Vpp (see Fig. 5b-d).

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