Colour Online: See the article online to view Scheme 1 and Fig. 2 in colour.
Dielectrophoretic manipulation and solubility of protein nanofibrils formed from crude crystallins
Version of Record online: 11 MAR 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Dielectrophoresis 2013
Volume 34, Issue 7, pages 1105–1112, April 2013
How to Cite
Domigan, L., Andersen, K. B., Sasso, L., Dimaki, M., Svendsen, W. E., Gerrard, J. A. and Castillo-León, J. (2013), Dielectrophoretic manipulation and solubility of protein nanofibrils formed from crude crystallins. ELECTROPHORESIS, 34: 1105–1112. doi: 10.1002/elps.201200495
- Issue online: 2 APR 2013
- Version of Record online: 11 MAR 2013
- Accepted manuscript online: 22 FEB 2013 03:52AM EST
- Manuscript Accepted: 26 DEC 2012
- Manuscript Revised: 21 DEC 2012
- Manuscript Received: 7 SEP 2012
- Danish Agency for Science Technology and Innovation. Grant Number: FSS 09-066053
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Figure S1. TEM images of protein nanofibres formed from crude crystallin proteins. Scale bars are 10 μm (A), and 1 μm (B).
Figure S2. Histogram showing length distribution of crystallin fibril preparation that was used for DEP experiments.
Figure S3. Design of the DEP microchip. The DEP microchip consisted of 5 pairs of electrodes, with large landing spaces located at the end of the chip for contact with the chip holder (A). A closer view of these electrodes (B and C), shows that each electrode pair is spaced 5 μm apart.
Figure S4. SEM images of electrode pair which gave the highest current values.
Figure S5. Control measurements of ThT fluorescence in the presence of different solvents. From left to right: Control) ThT buffer solution, A) Crystallin fibril buffer, B) Crystallin fibril buffer pH 7, C) Nanopure H2O, D) 100 mM PB pH 7.4, E) Ethanol, F) Isopropanol, G) Methanol, and H) Acetonitrile. Error bars represent the standard deviation of three replicates.
Figure S6. Representative TEM images of A) Crystallin fibrils resuspended in 50 mM PB pH 7.4, and B) Control PB grid. Scale bar is 0.2 μm.
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