Application of short-end injection procedure in CE


Correspondence: Professor Zdeněk Glatz, Department of Biochemistry, Faculty of Science and CEITEC, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic


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CE is well known for its many advantages, including the speed of the analyses; most of which can be accomplished in less than 10 min. However many potential fields of application such as pharmaceutical research and industry and clinical diagnostics require analyses of large numbers of samples and so need an even shorter analysis time to be practical. There are generally three main options for enhancing the throughput of electromigration methods – using microchip or multi-capillary systems or modifying the method preformed on single capillary instruments. The latter option will probably be the method of choice for most laboratories, since they are only equipped with classical commercial CE instrumentation. Although several approaches can be used – higher electric field, EOF modification, external pressure application during analysis, this article reviews one of the simplest and at the same time most used, the so-called short-end injection procedure.