These authors contributed equally to this article.
Micellar ordered structure effects on high-resolution CE-SSCP using Pluronic triblock copolymer blends
Article first published online: 24 JAN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 34, Issue 4, pages 518–523, February 2013
How to Cite
Hwang, H. S., Shin, G. W., Park, H. J., Ryu, C. Y. and Jung, G. Y. (2013), Micellar ordered structure effects on high-resolution CE-SSCP using Pluronic triblock copolymer blends. ELECTROPHORESIS, 34: 518–523. doi: 10.1002/elps.201200520
Colour Online: See the article online to view Fig. 5 in colour.
- Issue published online: 18 FEB 2013
- Article first published online: 24 JAN 2013
- Accepted manuscript online: 17 NOV 2012 05:54AM EST
- Manuscript Accepted: 11 OCT 2012
- Manuscript Revised: 24 SEP 2012
- Manuscript Received: 14 MAY 2012
- Agency for Defense Development. Grant Number: ADD-10-70-06-01
- High resolution;
- Pluronic polymer;
- Polymer blend;
- Polymer matrix
Pluronic F108 block copolymers have shown a great promise to achieve the desirable high resolution in the conformation-sensitive separation of ssDNA using CE-SSCP. However, fundamental understanding of the structures and properties of Pluronic matrix affecting the resolution is still limited. Unlike conventional gel-forming homopolymers, Pluronic F108 block copolymers are amphiphilic macromolecules consisting of poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) triblock copolymers, which are capable of forming a highly ordered micellar structure in aqueous solution. In this study, we have performed a series of experiments by blending different types of Pluronic polymers to control the formation of micelles and to study the correlation between separation and rheological characteristics of Pluronic gels affecting the resolution of CE-SSCP. Our experiments have been specifically designed to elucidate how the micellar structure affects the resolution of CE-SSCP upon altering the size uniformity and constituent homogeneity of the micelles. Our results suggest that uniformly sized micelle packing is the primary structural feature of Pluronic gel matrix for the high-resolution separation, while the size and constituent of the micelle themselves need to be considered as secondary factors.