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Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

Authors

  • Zuzana Svobodova,

    1. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
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  • Jana Kucerova,

    1. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
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  • Julien Autebert,

    1. Macromolecules and Microsystems in Biology and Medicine, Institute Curie, Paris, France
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  • Daniel Horak,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Czech Republic
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  • Lenka Bruckova,

    1. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
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  • Jean-Louis Viovy,

    1. Macromolecules and Microsystems in Biology and Medicine, Institute Curie, Paris, France
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  • Zuzana Bilkova

    Corresponding author
    1. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
    • Correspondence: Associate Professor Zuzana Bilkova, Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic

      E-mail: zuzana.bilkova@upce.cz

      Fax: +420-466-037-068

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  • Colour Online: See the article online to view Fig. 1 in colour.

Abstract

In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line.

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