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Communication
Detection of Sulfur Microparticles in Bacterial Cultures by Flow Cytometry
Article first published online: 6 JUL 2007
DOI: 10.1002/elsc.200720195
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
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Engineering in Life Sciences
Special Issue: Wolfgang Babel – Dedicated to his 70th Birthday
Volume 7, Issue 4, pages 403–407, July, 2007
Additional Information
How to Cite
Hübschmann, T., Vogt, C., Till, S., Rohwerder, T., Sand, W., Harms, H. and Müller, S. (2007), Detection of Sulfur Microparticles in Bacterial Cultures by Flow Cytometry. Engineering in Life Sciences, 7: 403–407. doi: 10.1002/elsc.200720195
Publication History
- Issue published online: 6 JUL 2007
- Article first published online: 6 JUL 2007
- Manuscript Accepted: 8 MAY 2007
- Manuscript Revised: 4 MAY 2007
- Manuscript Received: 2 APR 2007
- Abstract
- References
- Cited By
Keywords:
- Bacteria;
- Fow cytometry;
- Sulfur
Any interpretation of flow cytometric analyses of live bacteria grown under sulfate-reducing conditions requires specific caution since sulfur particles might cause errors in data interpretation. To prevent artefacts, distinct treatments of the sample are recommended.
Abstract
Repeated flow cytometric analyses of population shifts within a bacterial consortium growing under sulfate-reducing conditions revealed unexpected and quick changes in forward scatter vs. side scatter dot plot patterns within less than 30 minutes of exposure to air. These pattern changes proceeded differently when cells were cultivated either at 30 °C or 12 °C. It was found that prominent sub-distributions, clustering profoundly in size and number of events with time, represented sulfur particles and not microbial cells. Therefore, any interpretation of flow cytometric analyses of live bacteria grown under sulfate-reducing conditions requires specific caution since sulfur particles might cause errors in data interpretation. To prevent artefacts, distinct treatments of the sample are recommended.