Dedicated to Professor Wei-Shou Hu on the occasion of his 60th birthday.
Evaluation of criteria for bioreactor comparison and operation standardization for mammalian cell culture†
Article first published online: 9 JUL 2012
© 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Engineering in Life Sciences
Volume 12, Issue 5, pages 518–528, October 2012
How to Cite
Platas Barradas, O., Jandt, U., Minh Phan, L. D., Villanueva, M. E., Schaletzky, M., Rath, A., Freund, S., Reichl, U., Skerhutt, E., Scholz, S., Noll, T., Sandig, V., Pörtner, R. and Zeng, A.-P. (2012), Evaluation of criteria for bioreactor comparison and operation standardization for mammalian cell culture. Eng. Life Sci., 12: 518–528. doi: 10.1002/elsc.201100163
- Issue published online: 23 OCT 2012
- Article first published online: 9 JUL 2012
- Manuscript Accepted: 21 MAR 2012
- Manuscript Revised: 7 FEB 2012
- Manuscript Received: 26 AUG 2011
- BMBF. Grant Number: 0315275A
- Bioreactor geometry;
- Comparison criteria;
- Mammalian cell culture;
- Process standardization;
- Process transfer
Development of bioprocesses with mammalian cell culture deals with different bioreactor types and scales. The bioreactors might be intended for generation of cell inoculum and production, research, process development, validation, or transfer purposes. During these activities, not only the difficulty of up and downscaling might lead to failure of consistency in cell growth, but also the use of different bioreactor geometries and operation conditions. In such cases, criteria for bioreactor design and process transfer should be carefully evaluated in order to select appropriate cultivation parameters.
In this work, power input, mixing time, impeller tip speed, and Reynolds number have been compared systematically for the cultivation of the human cell line AGE1.HN within three partner laboratories using five different bioreactor systems. Proper operation ranges for the bioreactors were identified using the maximal cell-specific growth rate (μmax) as indicator. Common optimum values for process transfer criteria were found in these geometrically different bioreactors, in which deviations of μmax between cultivation systems can be importantly reduced. The data obtained in this work are used for process standardization and comparability of results obtained in different bioreactor systems, i.e. to guarantee lab-to-lab consistency for systems biology approaches using mammalian cells.