Effect of temperature on ethanol tolerance of thermotolerant Isshatchenkia orientalis IPE100

Authors

  • Yong-Jin Kwon,

    1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China
    2. Graduate University of the Chinese Academy of Sciences, Beijing, P. R. China
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  • Feng Wang,

    1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China
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  • Qian Li,

    1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China
    2. Graduate University of the Chinese Academy of Sciences, Beijing, P. R. China
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  • Chun-Zhao Liu

    Corresponding author
    • National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China
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Correspondence: Prof. Chun-Zhao Liu (czliu@home.ipe.ac.cn), Professor of Biochemical Engineering, National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, P. R. China

Abstract

Tolerance to high temperature and ethanol is a major factor in high-temperature bio-ethanol fermentation. The inhibitory effect of exogenously added ethanol (0–100 g L−1) on the growth of the newly isolated thermotolerant Issatchenkia orientalis IPE100 was evaluated at a range of temperatures (30–45°C). A generalized Monod equation with product inhibition was used to quantify ethanol tolerance, and it correlated well with the experimental data on microbial growth inhibition of ethanol at the temperatures of 30–45°C. The maximum inhibitory concentration of ethanol for growth (Pm) and toxic power (n) at the optimal growth temperature of 42°C were estimated to be 96.7 g L−1 and 1.23, respectively. The recently isolated thermotolerant I. orientalis IPE100 shows therefore a strong potential for the development of future high-temperature bio-ethanol fermentation technologies. This study provides useful insights into our understanding of the temperature-dependent inhibitory effects of ethanol on yeast growth.

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