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Figure S1. Characteristics for the synthesized silica: (A) BJH pore size distribution for the main cavities for the bare (▪) and modified-silica SGx6.0 ( ). (B, C) SEM micrographs for the bare silica (S) before (B) and after (C) be submitted to the immobilization conditions.

Figure S2. Solid state 29Si NMR spectrum for the silica modified by using GPTMS (S-Gx6.0 material)

Figure S3. Isoelectric point for pure silica, B. circulans (square), A oryzae (circles) and K. lactis (triangle) -galactosidades and silica (continues line).

Figure S4. Thermal stability at 55°C and pH 4.5 for Aspergillus oryzae -galactosidase before (▪) and after (○) amination process.

Figure S5. SDS-PAGE analysis of β-galactosidase from K. lactis (free or immobilized in glyoxyl-activated agarose support). Lane 1: molecular weight markers; lane 2: free β- galactosidase; lane 3: β-galactosidase immobilized in glyoxyl-activated agarose support

Figure S6. Lineweaver-Burk plot for β-galactosidase from B. circulans: free (▪), immbolized in glyoxyl-activated silica (▲, Bc-SGx1.0 ) and agarose (Δ, Bc-AGx)

Table S1. Some characteristics for the -galactosidades studied in this work

Table S2. Porous characteristics and aldehyde content for the different supports

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