Evaluation of the genotoxicity of Fusarium mycotoxin moniliformin in human peripheral blood lymphocytes
Article first published online: 19 FEB 2009
Copyright © 2009 Wiley-Liss, Inc.
Environmental and Molecular Mutagenesis
Volume 50, Issue 5, pages 431–434, June 2009
How to Cite
Çelik, M., Yılmaz, S., Aksoy, H., Ünal, F., Yüzbaşıoğlu, D. and Dönbak, L. (2009), Evaluation of the genotoxicity of Fusarium mycotoxin moniliformin in human peripheral blood lymphocytes. Environ. Mol. Mutagen., 50: 431–434. doi: 10.1002/em.20459
- Issue published online: 3 JUN 2009
- Article first published online: 19 FEB 2009
- Manuscript Accepted: 6 NOV 2008
- Manuscript Received: 28 OCT 2008
- KSU Research Fund. Grant Number: 2007/2-7
- chromosomal aberrations (CA);
- sister-chromatid exchange (SCE);
- micronucleus (MN) assay
Mycotoxins are fungal secondary metabolites that can be found in contaminated food and feed. There is some evidence to suggest that certain mycotoxins may be mutagenic. Here, we investigate the genotoxicity of the mycotoxin moniliformin (MON) (3-hydroxycyclobut-3-ene-1,2-dione) in human peripheral blood lymphocytes using chromosomal aberration (CA), sister-chromatid exchange (SCE), and micronucleus (MN) analysis. Lymphocyte cultures were treated for 48 h with six different concentrations of MON between 2.5 and 25 μM. CA, SCE, and MN frequencies were significantly increased in a dose-dependent manner compared with the negative control. The mitotic, replication, and cytokinesis-block proliferation indices were not affected by treatment with MON. The results provide evidence to demonstrate that MON can exert cytogenetic effects in human cells in culture. Environ. Mol. Mutagen. 2009. © 2009 Wiley-Liss, Inc.