See also: T Winters et al (June 2014)
STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis
Article first published online: 5 MAY 2014
© 2014 The Authors
The EMBO Journal
Volume 33, Issue 11, pages 1243–1255, 02 June 2014
How to Cite
The EMBO Journal (2014) 33: 1243–1255
- Issue published online: 2 JUN 2014
- Article first published online: 5 MAY 2014
- Manuscript Accepted: 28 MAR 2014
- Manuscript Revised: 26 MAR 2014
- Manuscript Received: 5 NOV 2013
- Swedish Cancer Society
- Swedish Research Council
- Torsten och Ragnar Söderbergs Stiftelser
- Karolinska Institutets
- Japan Society for the Promotion of Science. Grant Number: 25891015
- synaptonemal complex
Cohesion between sister chromatids in mitotic and meiotic cells is promoted by a ring-shaped protein structure, the cohesin complex. The cohesin core complex is composed of four subunits, including two structural maintenance of chromosome (SMC) proteins, one α-kleisin protein, and one SA protein. Meiotic cells express both mitotic and meiosis-specific cohesin core subunits, generating cohesin complexes with different subunit composition and possibly separate meiotic functions. Here, we have analyzed the in vivo function of STAG3, a vertebrate meiosis-specific SA protein. Mice with a hypomorphic allele of Stag3, which display a severely reduced level of STAG3, are viable but infertile. We show that meiocytes in homozygous mutant Stag3 mice display chromosome axis compaction, aberrant synapsis, impaired recombination and developmental arrest. We find that the three different α-kleisins present in meiotic cells show different dosage-dependent requirements for STAG3 and that STAG3-REC8 cohesin complexes have a critical role in supporting meiotic chromosome structure and functions.
Distinct cohesin complexes appear to mediate specialized chromosome organization function during meiosis. Analysis of a hypomorphic allele of one meiosis-specific cohesin subunit, STAG3/SA3, reveals its essential function in promoting chromosome synapsis during mouse meiosis.
- STAG3 is an essential subunit of the meiotic cohesin complex, required for gametogenesis and fertility.
- The three different α-kleisin subunits of the cohesin complex show different dosage-dependent requirements for STAG3.
- STAG3-REC8 containing cohesin complexes contribute to chromosome axis organization, chromosome synapsis, and recombination in meiotic cells.