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The EMBO Journal

Cover image for Vol. 30 Issue 24

December 14, 2011

Volume 30, Issue 24

Pages 4851–5022

  1. Have you seen?

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    3. Article
    4. Editor's Note
    5. Corrigendum
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      Human pluripotent stem cells decouple respiration from energy production (pages 4851–4852)

      Ng Shyh-Chang, Yuxiang Zheng, Jason W Locasale and Lewis C Cantley

      Version of Record online: 14 DEC 2011 | DOI: 10.1038/emboj.2011.436

      In human pluripotent stem cells, uncoupling protein 2 switches energy metabolism from respiration to glycolysis preventing their premature differentiation.

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      Building a double hexamer of DNA helicase at eukaryotic replication origins (pages 4853–4855)

      Karim Labib

      Version of Record online: 14 DEC 2011 | DOI: 10.1038/emboj.2011.437

      Yeast Cdt1 multitasks in recruitment, loading and activation of the Mcm2-7 helicase at replication origins—including a key role in bringing two helicases to a single origin recognition complex

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      Rotate into shape: MreB and bacterial morphogenesis (pages 4856–4857)

      Sven van Teeffelen and Zemer Gitai

      Version of Record online: 14 DEC 2011 | DOI: 10.1038/emboj.2011.430

      Several recent papers uncover interactions between the bacterial actin homologue, MreB, and components of the cell-wall synthesis machinery, revealing a tight interplay between cytoskeletal dynamics and cell-wall deposition.

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      The unmasking of novel unipotent stem cells in the mammary gland (pages 4858–4859)

      Jane E Visvader and Geoffrey J Lindeman

      Version of Record online: 8 NOV 2011 | DOI: 10.1038/emboj.2011.415

      Employing genetic lineage-tracing studies reveal the existence of novel, unipotent stem cells that serve the normal homeostasis of postnatal mammary epithelium. These findings add surprising complexity to the established stem cell hierarchy in the mammary epithelium.

  2. Article

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    3. Article
    4. Editor's Note
    5. Corrigendum
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      UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells (pages 4860–4873)

      Jin Zhang, Ivan Khvorostov, Jason S Hong, Yavuz Oktay, Laurent Vergnes, Esther Nuebel, Paulin N Wahjudi, Kiyoko Setoguchi, Geng Wang, Anna Do, Hea-Jin Jung, J Michael McCaffery, Irwin J Kurland, Karen Reue, Wai-Nang P Lee, Carla M Koehler and Michael A Teitell

      Version of Record online: 15 NOV 2011 | DOI: 10.1038/emboj.2011.401

      While studying metabolic fluxes in human pluripotent stem cells, this paper reveals UCP2 as metabolic switch from glycolysis to OXPHOS, facilitating early differentiation events.

      Corrected by:

      Corrigendum: UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

      Vol. 35, Issue 8, 899, Version of Record online: 18 APR 2016

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      FGF signalling inhibits neural induction in human embryonic stem cells (pages 4874–4884)

      Boris Greber, Philippe Coulon, Miao Zhang, Sören Moritz, Stefan Frank, Arnoldo José Müller-Molina, Marcos J Araúzo-Bravo, Dong Wook Han, Hans-Christian Pape and Hans R Schöler

      Version of Record online: 15 NOV 2011 | DOI: 10.1038/emboj.2011.407

      The role of FGF-signal inhibition in embryonic cell fate decisions remained controversial. This paper identifies OTX2 induction and downregulation of Oct4/Nanog as immediate downstream events that cooperate with Pax6 for efficient establishment of the neuroectodermal lineage.

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      Multiple Cdt1 molecules act at each origin to load replication-competent Mcm2–7 helicases (pages 4885–4896)

      Thomas J Takara and Stephen P Bell

      Version of Record online: 1 NOV 2011 | DOI: 10.1038/emboj.2011.394

      Replication initiation requires back-to-back loading of two Mcm2–7 helicases at a single origin. The replication licensing factor Cdt1 contributes to several discrete steps in this process, namely origin recruitment, chromatin loading, and conformational activation of Mcm2–7.

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      Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment (pages 4897–4907)

      Boris Pfander and John F X Diffley

      Version of Record online: 23 SEP 2011 | DOI: 10.1038/emboj.2011.345

      Cyclin-dependent kinase phosphorylation of the replication checkpoint mediator Rad9 controls its association with Dpb11, a key activator of the yeast ATR homologue Mec1, thus conferring cell-cycle dependence to checkpoint signalling.

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      Inhibition of autophagy by TAB2 and TAB3 (pages 4908–4920)

      Alfredo Criollo, Mireia Niso-Santano, Shoaib Ahmad Malik, Mickael Michaud, Eugenia Morselli, Guillermo Mariño, Sylvie Lachkar, Alexander V Arkhipenko, Francis Harper, Gérard Pierron, Jean-Christophe Rain, Jun Ninomiya-Tsuji, José M Fuentes, Sergio Lavandero, Lorenzo Galluzzi, Maria Chiara Maiuri and Guido Kroemer

      Version of Record online: 11 NOV 2011 | DOI: 10.1038/emboj.2011.413

      Expanding on recent crosstalk between autophagy and the NF-κB pathway, TAB2 and TAB3 are characterized as novel modulators of Beclin 1. Autophagic stimulation releases constitutive inhibition of Beclin 1 by TAB2/3 to initiate efficient autophagosome biogenesis.

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      Regulation of p53 stability and function by the deubiquitinating enzyme USP42 (pages 4921–4930)

      Andreas K Hock, Arnaud M Vigneron, Stephanie Carter, Robert L Ludwig and Karen H Vousden

      Version of Record online: 15 NOV 2011 | DOI: 10.1038/emboj.2011.419

      An RNAi screen identifies USP42 as a ubiquitin-specific isopeptidase that stabilizes p53 under transient stress conditions, and selectively enhances p53 function in the induction of cell-cycle arrest.

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      A widespread family of bacterial cell wall assembly proteins (pages 4931–4941)

      Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel and Jeff Errington

      Version of Record online: 30 SEP 2011 | DOI: 10.1038/emboj.2011.358

      While most steps of the bacterial cell wall synthesis pathway are well characterized, the identity of the enzymes that attach anionic polymers to peptidoglycan has been elusive. The LCP proteins are here shown to be likely candidates for this missing link.

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      Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry (pages 4942–4954)

      Adina Gerson-Gurwitz, Christina Thiede, Natalia Movshovich, Vladimir Fridman, Maria Podolskaya, Tsafi Danieli, Stefan Lakämper, Dieter R Klopfenstein, Christoph F Schmidt and Larisa Gheber

      Version of Record online: 18 NOV 2011 | DOI: 10.1038/emboj.2011.403

      The yeast kinesin-5 Cin8 can move bi-directionally on mitotic spindles and in-vitro assays provide insights into the molecular basis underlying this directional switching.

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      Proper synaptic vesicle formation and neuronal network activity critically rely on syndapin I (pages 4955–4969)

      Dennis Koch, Isabella Spiwoks-Becker, Victor Sabanov, Anne Sinning, Tamar Dugladze, Anne Stellmacher, Rashmi Ahuja, Julia Grimm, Susann Schüler, Anke Müller, Frank Angenstein, Tariq Ahmed, Alexander Diesler, Markus Moser, Susanne tom Dieck, Rainer Spessert, Tobias Maria Boeckers, Reinhard Fässler, Christian Andreas Hübner, Detlef Balschun, Tengis Gloveli, Michael Manfred Kessels and Britta Qualmann

      Version of Record online: 16 SEP 2011 | DOI: 10.1038/emboj.2011.339

      F-BAR domain proteins sense or induce membrane curvature and play a widespread role in intracellular trafficking events. This study characterizes syndapin I knockout mice at the neurological, cell biological and molecular levels and demonstrates that this F-BAR protein acts as membrane anchoring factor for dynamins during synaptic vesicle regeneration.

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      OCRL controls trafficking through early endosomes via PtdIns4,5P2-dependent regulation of endosomal actin (pages 4970–4985)

      Mariella Vicinanza, Antonella Di Campli, Elena Polishchuk, Michele Santoro, Giuseppe Di Tullio, Anna Godi, Elena Levtchenko, Maria Giovanna De Leo, Roman Polishchuk, Lisette Sandoval, Maria-Paz Marzolo and Maria Antonietta De Matteis

      Version of Record online: 4 OCT 2011 | DOI: 10.1038/emboj.2011.354

      Mutations in the PtdIns4,5P2 5-phosphatase OCRL cause the oculo-cerebro-renal Lowe syndrome. The molecular mechanisms underlying this condition involve defective early endosomal recycling of megalin, the receptor mediating protein reabsorption in the kidney.

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      Guanylate kinase domains of the MAGUK family scaffold proteins as specific phospho-protein-binding modules (pages 4986–4997)

      Jinwei Zhu, Yuan Shang, Caihao Xia, Wenning Wang, Wenyu Wen and Mingjie Zhang

      Version of Record online: 25 NOV 2011 | DOI: 10.1038/emboj.2011.428

      Membrane-associated guanylate kinases (MAGUK) family proteins contain an inactive guanylate kinase (GK) domain, whose function has been elusive. Here, this domain is revealed as a new type of phospho-peptide-binding module, in which the GMP-binding site has evolved to accommodate phospho-serines or -threonines.

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      PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro (pages 4998–5009)

      Takashi Fukaya and Yukihide Tomari

      Version of Record online: 25 NOV 2011 | DOI: 10.1038/emboj.2011.426

      The mechanism by which miRNAs induce silencing of gene expression remains a matter of much debate. This study shows that maximal silencing is dependent on multiple concerted interactions in the context of the full-length GW182 protein rather than single protein–protein associations.

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      Structure of the Shq1–Cbf5–Nop10–Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita (pages 5010–5020)

      Shuang Li, Jingqi Duan, Dandan Li, Shoucai Ma and Keqiong Ye

      Version of Record online: 25 NOV 2011 | DOI: 10.1038/emboj.2011.427

      Shq1 is required for the biogenesis of eukaryotic H/ACA ribonucleoproteins (RNPs) involved in telomere maintenance and pseudo-uridylation of rRNA and snRNA. This study describes the crystal structure of Shq1 in complex with three H/ACA RNP proteins, suggesting that Shq1 acts as an assembly chaperone of immature RNPs.

  3. Editor's Note

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    3. Article
    4. Editor's Note
    5. Corrigendum
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      miR-605 joins p53 network to form a p53:miR-605:Mdm2 positive feedback loop in response to stress (page 5021)

      Jiening Xiao, Huixian Lin, Xiaobin Luo, Xiaoyan Luo and Zhiguo Wang

      Version of Record online: 14 DEC 2011 | DOI: 10.1038/emboj.2011.463

  4. Corrigendum

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    3. Article
    4. Editor's Note
    5. Corrigendum
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      Extracellular heat shock protein-90α: linking hypoxia to skin cell motility and wound healing (page 5022)

      Wei Li, Yong Li, Shengxi Guan, Jianhua Fan, Chieh-Fang Cheng, Alexandra M Bright, Cindi Chinn, Mei Chen and David T Woodley

      Version of Record online: 14 DEC 2011 | DOI: 10.1038/emboj.2011.420

      This article corrects:

      Extracellular heat shock protein-90α: linking hypoxia to skin cell motility and wound healing

      Vol. 26, Issue 5, 1221–1233, Version of Record online: 15 FEB 2007

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