These authors contributed equally to this work.
Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
Article first published online: 4 MAR 2011
Copyright © 2011 EMBO Molecular Medicine
EMBO Molecular Medicine
Volume 3, Issue 4, pages 201–207, April 2011
How to Cite
Mamedov, I. Z., Britanova, O. V., Bolotin, D. A., Chkalina, A. V., Staroverov, D. B., Zvyagin, I. V., Kotlobay, A. A., Turchaninova, M. A., Fedorenko, D. A., Novik, A. A., Sharonov, G. V., Lukyanov, S., Chudakov, D. M. and Lebedev, Y. B. (2011), Quantitative tracking of T cell clones after haematopoietic stem cell transplantation. EMBO Mol Med, 3: 201–207. doi: 10.1002/emmm.201100129
- Issue published online: 1 APR 2011
- Article first published online: 4 MAR 2011
- Accepted manuscript online: 16 FEB 2011 04:40AM EST
- Manuscript Accepted: 10 FEB 2011
- Manuscript Revised: 9 FEB 2011
- Manuscript Received: 20 DEC 2010
- ankylosing spondylitis;
Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.