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Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles—a role of the endocytic–exocytic pathway

  1. Nicola Bauer1,
  2. Michaela Wilsch-Bräuninger2,†,
  3. Jana Karbanová1,†,
  4. Ana-Violeta Fonseca1,†,
  5. Doreen Strauss1,
  6. Daniel Freund1,
  7. Christoph Thiele2,
  8. Wieland B. Huttner2,
  9. Martin Bornhäuser3,4,
  10. Denis Corbeil1,3,*

Article first published online: 18 MAY 2011

DOI: 10.1002/emmm.201100147

Issue

EMBO Molecular Medicine

EMBO Molecular Medicine

Volume 3, Issue 7, pages 398–409, July 2011

Additional Information

How to Cite

Bauer, N., Wilsch-Bräuninger, M., Karbanová, J., Fonseca, A.-V., Strauss, D., Freund, D., Thiele, C., Huttner, W. B., Bornhäuser, M. and Corbeil, D. (2011), Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles—a role of the endocytic–exocytic pathway. EMBO Mol Med, 3: 398–409. doi: 10.1002/emmm.201100147

Author Information

  1. 1

    Tissue Engineering Laboratories (BIOTEC), Technische Universität Dresden, Dresden, Germany

  2. 2

    Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany

  3. 3

    DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany

  4. 4

    Medical Clinic and Polyclinic I, University Hospital Carl Gustav Carus, Dresden, Germany

  1. †

    MWB, JK and A-VF should be considered joint second authors.

*Tel: +49 (0) 351 463 40118, Fax: +49 (0) 351 463 40244

  1. †

    MWB, JK and A-VF should be considered joint second authors.

Publication History

  1. Issue published online: 6 JUL 2011
  2. Article first published online: 18 MAY 2011
  3. Manuscript Accepted: 11 APR 2011
  4. Manuscript Revised: 25 MAR 2011
  5. Manuscript Received: 18 OCT 2010

Funded by

  • CT. Grant Number: TRR83 #12
  • MB. Grant Number: SFB655 B2
  • WBH. Grant Number: TRR83 #6; SFB655 A2
  • DC. Grant Numbers: TRR83 #6; SFB655 B3, CO298/5-1

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Keywords:

  • CD133;
  • cell differentiation;
  • exosome;
  • haematopoietic stem cell;
  • lipid raft

Abstract

The differentiation of stem cells is a fundamental process in cell biology and understanding its mechanism might open a new avenue for therapeutic strategies. Using an ex vivo co-culture system consisting of human primary haematopoietic stem and progenitor cells growing on multipotent mesenchymal stromal cells as a feeder cell layer, we describe here the exosome-mediated release of small membrane vesicles containing the stem and cancer stem cell marker prominin-1 (CD133) during haematopoietic cell differentiation. Surprisingly, this contrasts with the budding mechanism underlying the release of this cholesterol-binding protein from plasma membrane protrusions of neural progenitors. Nevertheless, in both progenitor cell types, protein–lipid assemblies might be the essential structural determinant in the release process of prominin-1. Collectively, these data support the concept that prominin-1-containing lipid rafts may host key determinants necessary to maintain stem cell properties and their quantitative reduction or loss may result in cellular differentiation.

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