These authors contributed equally to this work.
MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction
Article first published online: 22 JUL 2013
Copyright © 2013 EMBO Molecular Medicine
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EMBO Molecular Medicine
Volume 5, Issue 9, pages 1402–1414, September 2013
How to Cite
Li, J., Li, J., Liu, X., Qin, S., Guan, Y., Liu, Y., Cheng, Y., Chen, X., Li, W., Wang, S., Xiong, M., Kuzhikandathil, E. V., Ye, J.-H. and Zhang, C. (2013), MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction. EMBO Mol Med, 5: 1402–1414. doi: 10.1002/emmm.201201900
- Issue published online: 3 SEP 2013
- Article first published online: 22 JUL 2013
- Manuscript Accepted: 17 JUN 2013
- Manuscript Revised: 2 MAY 2013
- Manuscript Received: 17 AUG 2012
- NIH Grants, HL095707, R21HL109656 and R21NR013876, CZ, PI
- NIH Grant, AA016964, J. E, PI
- alcohol addiction;
- dopamine receptor D1;
Alcohol addiction is a major social and health concern. Here, we determined the expression profile of microRNAs (miRNAs) in the nucleus accumbens (NAc) of rats treated with alcohol. The results suggest that multiple miRNAs were aberrantly expressed in rat NAc after alcohol injection. Among them, miR-382 was down-regulated in alcohol-treated rats. In both cultured neuronal cells in vitro and in the NAc in vivo, we identified that the dopamine receptor D1 (Drd1) is a direct target gene of miR-382. Via this target gene, miR-382 strongly modulated the expression of DeltaFosB. Moreover, overexpression of miR-382 significantly attenuated alcohol-induced up-regulation of DRD1 and DeltaFosB, decreased voluntary intake of and preference for alcohol and inhibited the DRD1-induced action potential responses. The results indicated that miRNAs are involved in and may represent novel therapeutic targets for alcoholism.