These authors contributed equally to this work.
Strategy for the creation of clinical grade hESC line banks that HLA-match a target population
Article first published online: 19 NOV 2012
Copyright © 2013 EMBO Molecular Medicine
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EMBO Molecular Medicine
Volume 5, Issue 1, pages 10–17, January 2013
How to Cite
Jacquet, L., Stephenson, E., Collins, R., Patel, H., Trussler, J., Al-Bedaery, R., Renwick, P., Ogilvie, C., Vaughan, R. and Ilic, D. (2013), Strategy for the creation of clinical grade hESC line banks that HLA-match a target population. EMBO Mol Med, 5: 10–17. doi: 10.1002/emmm.201201973
- Issue published online: 3 JAN 2013
- Article first published online: 19 NOV 2012
- Manuscript Accepted: 9 OCT 2012
- Manuscript Revised: 4 OCT 2012
- Manuscript Received: 3 SEP 2012
- Funded Access
- clinical grade stem cells;
- clinical grade human embryonic stem cell validation and banking;
- HLA typing;
- human embryonic stem cells;
- single cell analyses
Here, we describe a pre-derivation embryo haplotyping strategy that we developed in order to maximize the efficiency and minimize the costs of establishing banks of clinical grade hESC lines in which human leukocyte antigen (HLA) haplotypes match a significant proportion of the population. Using whole genome amplification followed by medium resolution HLA typing using PCR amplification with sequence-specific primers (PCR-SSP), we have typed the parents, embryos and hESC lines from three families as well as our eight clinical grade hESC lines and shown that this technical approach is rapid, reliable and accurate. By employing this pre-derivation strategy where, based on HLA match, embryos are selected for a GMP route on day 3–4 of development, we would have drastically reduced our cGMP laboratory running costs.