MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways
Article first published online: 3 JUN 2013
Copyright © 2013 EMBO Molecular Medicine
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EMBO Molecular Medicine
Volume 5, Issue 7, pages 1017–1034, July 2013
How to Cite
Cheng, H. S., Sivachandran, N., Lau, A., Boudreau, E., Zhao, J. L., Baltimore, D., Delgado-Olguin, P., Cybulsky, M. I. and Fish, J. E. (2013), MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways. EMBO Mol Med, 5: 1017–1034. doi: 10.1002/emmm.201202318
- Issue published online: 3 JUL 2013
- Article first published online: 3 JUN 2013
- Manuscript Accepted: 29 APR 2013
- Manuscript Revised: 26 APR 2013
- Manuscript Received: 1 DEC 2012
- Heart and Stroke Foundation of Ontario and the Canadian Institutes of Health Research
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Table SI. Primers used for qRT-PCR.
Figure S1. TNF-α induces miR-146a and miR-146b expression.
Figure S2. Over-expression of miR-146a inhibits monocyte adhesion to IL-1β-treated bovine aortic endothelial cells (BAEC).
Figure S3. miR-146a does not directly regulate EGR-3.
Figure S4. A potential miR-146 binding site in HuR is highly conserved across species.
Figure S5. miR-146 controls the expression of HuR mRNA.
Figure S6. HuR knock-down represses THP-1 adhesion to TNF-α-treated endothelial cells.
Figure S7. Predicted AU-rich elements (AREs) in the 3′ UTRs of genes involved in endothelial activation.
Figure S8. HuR binds to VCAM-1 and MCP-1 mRNA but does not regulate the induction of these genes by IL-1β.
Figure S9. The miR-146 targets, HuR and TRAF6, have divergent effects on the induction of inflammatory genes.
Figure S10. MicroRNAs previously implicated in regulating inflammation are not appreciably altered in miR-146a−/− mice.
Figure S11. Expression of eNOS is modestly decreased in miR-146a−/− mice.
Figure S12. KLF2 mRNA is bound by HuR and knock-down of HuR leads to increased levels of KLF2 transcripts.
Figure S13. Schematic of a miR-146 feedback loop that controls endothelial activation.
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