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Figure S1. Detection of lymph node metastsis.

Figure S2. TGF-β does not affect apoptosis and cell cycle of B16 cells.

Figure S3. Oral administration of EW-7197 suppresses melanoma and LN metastases with enhanced CTL activity.

Figure S4. Oral administration of EW-7197 inhibits TGF-β signalling and downregulates Smad4 in spleen cells of melanoma-bearing mice.

Figure S5. Oral administration of LY-2157299 inhibits TGF-β signalling and downregulates Smad4 in dLN cells of melanoma-bearing mice.

Figure S6. LY-2157299 induces Smad4 ubiquitination in dLN cells of melanoma-bearing mice.

Figure S7. R-Smads are not ubiquitinated in dLN cells of EW-7197-treated melanoma-bearing mice.

Figure S8. Smurf1 and Smurf2 are not involved in degradation of Smad4 by ALK5 inhibition.

Figure S9. T-cell-specific Smad4 deletion suppresses melanoma and LN metastases with enhanced CTL activity.

Figure S10. Upregulation of Eomes in CD8+ T cells by ALK5 inibition or T-cell-specific Smad4 deletion.

Figure S11. Eomes is not expressed in CD4+ cells in the dLNs of melanoma-bearing mice.

Figure S12. Characterization of TILs.

Figure S13. Deletion of CD8+ T cells, CD4+ T cells, NK cells in vivo.

Figure S14. T-cell-specific Smad4 deletion.

Figure S15. Effect of Smad4 on PMA/ionomycin-stimulated CD8+ T cells.

Table S1. Primer sequences for quantitative RT-PCR.

Table S2. Primer sequences for the proximal promoter regions of Eomes.

Table S3. Primer sequences for ChIP.

emmm201302524-SourceData-Fig2.pdfPDF document1878KSource Data for Figure 2
emmm201302524-SourceData-Fig3.pdfPDF document1878KSource Data for Figure 3
emmm201302524-SourceData-FigS14.pdfPDF document320KSource Data for Figures S7 and S14

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