Ret inhibition decreases growth and metastatic potential of estrogen receptor positive breast cancer cells
Article first published online: 19 JUL 2013
Copyright © 2013 EMBO Molecular Medicine
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EMBO Molecular Medicine
Volume 5, Issue 9, pages 1335–1350, September 2013
How to Cite
Gattelli, A., Nalvarte, I., Boulay, A., Roloff, T. C., Schreiber, M., Carragher, N., Macleod, K. K., Schlederer, M., Lienhard, S., Kenner, L., Torres-Arzayus, M. I. and Hynes, N. E. (2013), Ret inhibition decreases growth and metastatic potential of estrogen receptor positive breast cancer cells. EMBO Mol Med, 5: 1335–1350. doi: 10.1002/emmm.201302625
- Issue published online: 3 SEP 2013
- Article first published online: 19 JUL 2013
- Manuscript Accepted: 17 JUN 2013
- Manuscript Revised: 29 MAY 2013
- Manuscript Received: 11 FEB 2013
- Novartis Research Foundation
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Figure S1. A. Validation of Ret antibody for immunohistochemical (IHC) staining of paraffin sections.
Figure S2. A. Western bot (WB) analysis on lysates from MCF7/Aro, MCF7 and T47D cells was carried out with a Ret antibody (anti-human Ret antibody from Cell Signalling) in the upper panels.
Figure S3. A. Microarray slides were co-hybridized with cDNA from 6-day treated MCF7/Aro cells cultured in the 6 indicated conditions (as in Fig 2F).
Figure S4. A. MCF7 cells were pre-incubated with DMSO or NVP-AST487 (100 nM), then treated 15 min with IL6 (100 ng/ml) or GDNF (10 ng/ml).
Figure S5. A. Groups of J110-tumour bearing mice were randomized and treated once daily with vehicle (10%EtOH in corn oil) or tamoxifen (100 µg/day) for 3 weeks.
Figure S6. A, B. Tumours from mice treated as described in Fig 5C were harvested 8 hours after the final treatment.
Table S1. Correlation of Ret-score with established clinical and histopathological parameters of breast cancer patients.
Table S2. Correlation of Ret score with molecular subtypes of the analysed breast cancer patients.
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