See accompanying article 10.1002/emmm.201303483
Instruction of haematopoietic lineage choices, evolution of transcriptional landscapes and cancer stem cell hierarchies derived from an AML1-ETO mouse model
Version of Record online: 4 OCT 2013
© 2013 The Authors. Published by John Wiley and Sons, Ltd on behalf of EMBO
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EMBO Molecular Medicine
Volume 5, Issue 12, pages 1804–1820, December 2013
How to Cite
Cabezas-Wallscheid, N., Eichwald, V., de Graaf, J., Löwer, M., Lehr, H.-A., Kreft, A., Eshkind, L., Hildebrandt, A., Abassi, Y., Heck, R., Dehof, A. K., Ohngemach, S., Sprengel, R., Wörtge, S., Schmitt, S., Lotz, J., Meyer, C., Kindler, T., Zhang, D.-E., Kaina, B., Castle, J. C., Trumpp, A., Sahin, U. and Bockamp, E. (2013), Instruction of haematopoietic lineage choices, evolution of transcriptional landscapes and cancer stem cell hierarchies derived from an AML1-ETO mouse model. EMBO Mol Med, 5: 1804–1820. doi: 10.1002/emmm.201302661
- Issue online: 2 DEC 2013
- Version of Record online: 4 OCT 2013
- Manuscript Revised: 28 AUG 2013
- Manuscript Accepted: 28 AUG 2013
- Manuscript Received: 20 FEB 2013
- Deutsche Krebshilfe (10-1982-BoI-II) and Deutsche Forschungsgemeinschaft (DFG) (Forschergruppe 527)
- Helmholtz Society, Dietmar Hopp Foundation and DFG funded Sonderforschungsbereich (SFB) 873
- German Research Foundation. Grant Number: DFG; FB636/A4
- Federal Ministry of Education and Research. Grant Number: BMFT NGFN/SP10
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Figure S1. (A) The R26/AE mouse model directs GFP transgene expression to a subset of HSC and blood cell progenitors. (B) Median fluorescence of GFP+ haematopoietic stem cells and lineage-restricted blood cell progenitors.
Figure S2. Peripheral blood parameters in control and AE-expressing mice.
Figure S3. Histological sections through haematopoietic organs from control and AE-expressing mice.
Figure S4. Flow cytometric analysis of extramedullary haematopoiesis in control and AE-expressing spleens.
Figure S5. Flow cytometric analysis of control and AE-expressing mice reveals red blood cell lineage maturation defects and the expansion of granulocytes.
Figure S6. Flow cytometric analysis of control and AE-expressing mice reveals B and T cell maturation defects.
Figure S7. AE expression does not alter the haematopoietic stem cell compartment.
Figure S8. AE expression does not change CLP but alters the CMP, GMP and MEP pools of lineage-restricted progenitors.
Figure S9. Analysis of GFP− and GFP+ LT−, ST-HSC and CLP populations reveals no significant pool size differences between control and AE expressing mice.
Figure S10. Relative percentages of GFP+ but not GFP− GMP and MEP pools are altered in AE-expressing mice.
Figure S11. Immature granulocytes are increased in the BM of long-term AE-expressing mice.
Figure S12. Long-term AE expression does not alter the pool of LT- and ST-HSC in leukaemic mice.
Figure S13. AE expression alters the relative percentages of CMP, GMP and MEP.
Figure S14. Analysis of GFP− and GFP+ LT−, ST-HSC and CLP populations reveals no significant differences between control and leukaemic mice.
Figure S15. Relative percentages of GFP+ but not GFP− CMP, GMP and MEP pools are altered in leukaemic mice.
Figure S16. Flow cytometric analysis of BM granulocytes reveals the reduction of immature myeloid cells in two previously leukaemic mice following the DOX switch.
Figure S17. Post reconstitution flow cytometric analysis demonstrates that L-HSC reconstitute both lympho- and myelopoiesis whereas L-GMP only reconstitute myelopoiesis.
Figure S18. Flow cytometric analysis reveals differences in granulocytic maturation between L-HSC- and L-GMP-reconstituted mice.
|emmm201302625-sm-0003-SuppTab-S1.xls||3358K||Table S1. Table S1 and Table S2: Whole transcriptome and mRNA profiles of Ctrl-, ST- and L-GMP populations are provided in Table S1 and Table S2.|
|emmm201302625-sm-0004-SuppTab-S2.xls||5180K||Table S2. Table S1 and Table S2: Whole transcriptome and mRNA profiles of Ctrl-, ST- and L-GMP populations are provided in Table S1 and Table S2.|
|emmm201302625-sm-0005-SuppTab-S3.xls||1948K||Table S3. Table S3 contains lists of transcripts that were specifically increased, further upregulated, newly transcribed, specifically downregulated, further decreased or completely extinguished in L-GMP cells.|
|emmm201302625-sm-0006-SuppTab-S4.xls||1035K||Table S4. Table S4 contains the list of human orthologous genes corresponding to the top 150 transcripts that were highly upregulated or exclusively expressed in murine ST-GMP and in murine L-GMP indicating the rank in the gene list, the metric score and the individual enrichment score found in a cohort of 61 t(8;21) CBF AML patients.|
|emmm201302625-sm-0007-SuppTab-S5.xls||80K||Table S5. Table S5 contains the list of 17 human genes that were found to be on average two fold or more increased in a group of 61 t(8;21) patients as compared to a cohort of 69 healthy donors.|
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