Construction of engineering microorganism degrading chlorophenol efficiently by protoplast fusion technique

Authors

  • Honglei Chen,

    1. State Key Laboratory of Pulp and Paper Engineering, Light Industry and Food Department, South China University of Technology, Guangzhou, Guangdong, China
    2. Key Laboratory of Pulp and Paper Science and Technology of Ministry of Education, Light Industry and Chemistry Department, Shan Dong Polytechnic University, Jinan, Shandong, China
    Search for more papers by this author
  • Huaiyu Zhan,

    1. State Key Laboratory of Pulp and Paper Engineering, Light Industry and Food Department, South China University of Technology, Guangzhou, Guangdong, China
    Search for more papers by this author
  • Yuancai Chen,

    Corresponding author
    • State Key Laboratory of Pulp and Paper Engineering, Light Industry and Food Department, South China University of Technology, Guangzhou, Guangdong, China
    Search for more papers by this author
  • Shiyu Fu

    1. State Key Laboratory of Pulp and Paper Engineering, Light Industry and Food Department, South China University of Technology, Guangzhou, Guangdong, China
    Search for more papers by this author

chenyc@scut.edu.cn (for correspondence)

Abstract

Protoplast fusion is an important technique for engineering microbial strains. It has been used to combine genes from different organisms to create new strains with desired properties. This investigation was aimed to construct a new strain with an ability of degrading chlorophenols efficiently by protoplast fusion between Pseudomonas putida and Psathyrella candolleana. Twenty recombinant strains were screened and used to degrade pentachlorophenol (PCP) synthetic wastewater in order to determine their capabilities of chlorophenol degradation. To rapidly and accurately measure PCP content in synthetic wastewater after treatment by different strains, a new method quantifying PCP was proposed based on derivative spectroscopy. Chlorophenol biodegradability of different strains could be measured rapidly according to the new method. The results showed that PCP degradation % of four recombinant strains viz., Xz 6-1, Xz 6-3, Xz 6-5, Xz 8-2 were 78.98%, 69.12%, 31.40%, 46.26%, respectively, and that of parent strain (Pseudomonas putida.) was 57.27%, which indicated that the degradation % of Xz 6-1 is the highest and improved by 21.71% compared with Pseudomonas putida. Furthermore, the colony morphologies and microscopic feature of Xz 6-1 and Pseudomonas putida were compared in this article. © 2012 American Institute of Chemical Engineers Environ Prog, 32: 443–448, 2013

Ancillary