Enzymatic decolorization of Orange II: Optimization by response surface methodology and pathway

Authors

  • Anping Zhang,

    1. International Joint Research Center for Persistent Toxic Substances, Zhejiang University of Technology, Hangzhou, People's Republic of China
    Search for more papers by this author
  • Li Fang,

    1. International Joint Research Center for Persistent Toxic Substances, Zhejiang University of Technology, Hangzhou, People's Republic of China
    Search for more papers by this author
  • Junliang Wang,

    Corresponding author
    • International Joint Research Center for Persistent Toxic Substances, Zhejiang University of Technology, Hangzhou, People's Republic of China
    Search for more papers by this author
  • Weiping Liu

    1. International Joint Research Center for Persistent Toxic Substances, Zhejiang University of Technology, Hangzhou, People's Republic of China
    2. MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, People's Republic of China
    Search for more papers by this author

upupwang@zjut.edu.cn (for correspondence)

Abstract

Statistics-based experimental design with response surface methodology (RSM) was applied to optimize the operating conditions in decolorization of Orange II by horseradish peroxidase (HRP) in the presence of H2O2. Four critical factors (i.e., HRP concentration, H2O2 concentration, pH, and reaction time) were used for the optimization of enzymatic decolorization using RSM. The optimized conditions of the enzymatic degradation of Orange II were as follows: HRP concentration, 0.80 U/mL; H2O2 concentration, 0.70 mM; pH, 9.0; reaction time, 40 min. The maximal decolorization efficiency of 95.83% was achieved at the optimized conditions. The analysis of UV–vis spectra and electrospray ionization–time of flight–mass spectrometry indicated that both decomposition and coupling reaction occurred in the enzymatic decolorization. © 2012 American Institute of Chemical Engineers Environ Prog, 32: 294-301, 2013

Ancillary