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Quick assessment of cytotoxins effect on Daphnia magna using in vivo fluorescence microscopy

Authors

  • Vera V. Teplova,

    1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow Region, Pushchino, 142290 Russia
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  • Zhanna I. Andreeva-Kovalevskaya,

    1. Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia
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  • Elena V. Sineva,

    Corresponding author
    1. Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia
    Current affiliation:
    1. Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego, La Jolla, California 92093-0703, USA.
    • Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
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  • Alexander S. Solonin

    1. Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia
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Abstract

A novel approach to contaminant toxicity screening is proposed. The use of fluorescent microscopy with fluorescent dyes allows for assessing intoxication of Daphnia magna tissues, at various stages of exposure, to contaminants present in water. As shown, D. magna may not only be used as a test species in toxicity tests based on its lethality, but due to its translucency and application of fluorescent probes, separate steps of its intoxication and dying can be visualized. Using a variety of fluorescent probes, the present study also contributes to a better understanding of the toxicity mechanisms. Environ. Toxicol. Chem. 2010;29:1345–1348. © 2010 SETAC

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