Formation of DNA adducts by reactive chemicals or their metabolites are often a precursor of mutagenesis and other adverse effects. Studies in juvenile channel catfish (Ictaluruspunctatus) were conducted to determine the dose–response, kinetics of formation, and persistence of S-[2-(N7-guanyl)ethyl]glutathione hepatic–DNA adducts following a 4-h in vivo aqueous exposure to ethylene dichloride (EDC) at several dose levels. S-[2-(N7-guanyl)ethyl] glutathione adducts were detectable in liver tissue after 2 h of exposure and were still detectable three weeks after a single pulse exposure (detection limit = ∼10 fmol, ∼1 DNA adduct in 107 bases). Pretreatment of catfish with the glutathione-depleting agent diethylmaleate significantly reduced the level of tissue glutathione levels and, as a result, DNA adducts were not detected in pretreated fish. Catfish may serve as a useful sentinel species for detecting DNA-reactive chemicals in aquatic systems. Environ. Toxicol. Chem. 2010;29:1537–1544. © 2010 SETAC
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