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2,3,4,7,8-pentachlorodibenzofuran is a more potent cytochrome P4501A inducer than 2,3,7,8-tetrachlorodibenzo-p-dioxin in herring gull hepatocyte cultures

Authors

  • Jessica C. Hervé,

    1. Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    2. National Wildlife Research Centre, Environment Canada, Ottawa, Ontario K1S 5B6, Canada
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  • Doug L.D. Crump,

    1. National Wildlife Research Centre, Environment Canada, Ottawa, Ontario K1S 5B6, Canada
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  • Kristina K. McLaren,

    1. National Wildlife Research Centre, Environment Canada, Ottawa, Ontario K1S 5B6, Canada
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  • John P. Giesy,

    1. Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada
    2. Zoology Department and Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
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  • Matthew J. Zwiernik,

    1. Zoology Department and Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
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  • Steven J. Bursian,

    1. Zoology Department and Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
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  • Sean W. Kennedy

    Corresponding author
    1. Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    2. National Wildlife Research Centre, Environment Canada, Ottawa, Ontario K1S 5B6, Canada
    • Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
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Abstract

Concentration-dependent effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofuran (PeCDF), and 2,3,7,8-tetrachlorodibenzofuran (TCDF) on cytochrome P4501A (CYP1A) induction were determined in primary cultures of embryonic herring gull (Larus argentatus) hepatocytes exposed for 24 h. Based on the concentration that induced 50% of the maximal response (EC50), the relative potencies of TCDD and TCDF did not differ by more than 3.5-fold. However, also based on the EC50, PeCDF was 40-fold, 21-fold, and 9.8-fold more potent for inducing ethoxyresorufin-O-deethylase (EROD) activity, CYP1A4 mRNA expression, and CYP1A5 mRNA expression than TCDD, respectively. The relative CYP1A-inducing potencies of PeCDF and of other dioxin-like chemicals (DLCs) in herring gull hepatocytes (HEH RePs), along with data on concentrations of DLCs in Great Lakes herring gull eggs, were used to calculate World Health Organization toxic equivalent (WHO-TEQ) concentrations and herring gull embryonic hepatocyte toxic equivalent (HEH-TEQ) concentrations. The analysis indicated that, when using avian toxic equivalency factors (TEFs) recommended by the WHO, the relative contribution of TCDD (1.1–10.2%) to total WHO-TEQ concentration was higher than that of PeCDF (1.7–2.9%). These results differ from the relative contribution of TCDD and PeCDF when HEH RePs were used; PeCDF was a major contributor (36.5–52.9%) to total HEH-TEQ concentrations, whereas the contribution by TCDD (1.2–10.3%) was less than that of PeCDF. The WHO TEFs for avian species were largely derived from studies with the domestic chicken (Gallus gallus domesticus). The findings of the present study suggest that it is necessary to determine the relative potencies of DLCs in wild birds and to re-evaluate their relative contributions to the biochemical and toxic effects previously reported in herring gulls and other avian species. Environ. Toxicol. Chem. 2010;29:2088–2095. © 2010 SETAC

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