Joint action of pollutant combinations (pesticides and metals) on survival (LC50 values) and acetylcholinesterase activity of Tigriopus brevicornis (Copepoda, Harpacticoida)

Authors

  • Joëlle Forget,

    Corresponding author
    1. Laboratoire de Physiologie des ětres marins, Institut Océanographique, 195, rue Saint-Jacques, F-75005 Paris, France
    2. DEL Polluants chimiques, IFREMER, rue de I'lle d'Yeu, F-44311 Nantes Cédex 03, France
    • Laboratoire de Physiologie des ětres marins, Institut Océanographique, 195, rue Saint-Jacques, F-75005 Paris, France
    Search for more papers by this author
  • Jean-François Pavillon,

    1. Laboratoire de Physiologie des ětres marins, Institut Océanographique, 195, rue Saint-Jacques, F-75005 Paris, France
    Search for more papers by this author
  • Benoǐt Beliaeff,

    1. DEL Applications Opérationnelles, IFREMER, rue de I'lle d'Yeu, F-44311 Nantes Cédex 03, France
    Search for more papers by this author
  • Gilles Bocquené

    1. DEL Polluants chimiques, IFREMER, rue de I'lle d'Yeu, F-44311 Nantes Cédex 03, France
    Search for more papers by this author

Abstract

The joint toxicity of nine binary mixtures of a metal (arsenic, copper, or cadmium) and a pesticide (carbofuran, dichlorvos, or malathion) was determined in the marine microcrustacean Tigriopus brevicornis (Müller) (Copepoda) by 96-h LC50 tests and measurement of acetylcholinesterase (AChE) inhibition. Acetylcholinesterase is used in the marine coastal environment as a biomarker to evaluate exposure to neurotoxic pollutants, including organophosphorous (OP) and carbamate (C) insecticides and most metals. A toxic unit (TU) approach was used to test the response addition model for mixtures of chemicals with different action modes. Studies of mixtures showed synergistic lethal effects in all cases (the strongest acute effects being observed in coppermalathion, cadmium-malathion, dichlorvos-malathion, and cadmium-dichlorvos combinations). At the sublethal level, the presence of the three metals tested seemed to enhance the inhibitory effects of certain OP and C insecticides.

Ancillary