Toxicity of aqueous C70-gallic acid suspension in Daphnia magna

Authors

  • Brandon C. Seda,

    Corresponding author
    1. Institute of Environmental Toxicology, Clemson University, Pendleton, South Carolina, USA
    • Institute of Environmental Toxicology, Clemson University, Pendleton, South Carolina, USA.

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  • Pu-Chun Ke,

    1. Institute of Environmental Toxicology, Clemson University, Pendleton, South Carolina, USA
    2. Department of Physics and Astronomy, Clemson University, Clemson, South Carolina, USA
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  • Andrew S. Mount,

    1. Institute of Environmental Toxicology, Clemson University, Pendleton, South Carolina, USA
    2. Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA
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  • Stephen J. Klaine

    1. Institute of Environmental Toxicology, Clemson University, Pendleton, South Carolina, USA
    2. Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA
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  • Presented at Nano 2010: International Conference on the Environmental Effects of Nanomaterials, Clemson University, August 2010.

Abstract

The present study assessed the toxic effects of stable aqueous colloidal suspensions of gallic-acid-stabilized C70 fullerene on Daphnia magna. The suspensions were stabilized through noncovalent surface modification with gallic acid. In addition to whole-organism responses, changes in antioxidative processes in D. magna were quantified. Acute toxicity was observed with 96LC50 for C70-gallic acid of 0.4 ± 0.1 mg/L C70. Daphnia magna fecundity was significantly reduced in 21-d bioassays at C70-gallic aqcid concentrations below quantifiable limits. Antioxidant enzyme activities of glutathione peroxidase and superoxide dismutase as well as lipid peroxidation suggested that exposed organisms experienced oxidative stress. Microscopic techniques used to determine cellular toxicity via apoptosis proved unsuccessful. Environ. Toxicol. Chem. 2012;31:215–220. © 2011 SETAC

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