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The role of the characteristics of humic substances in binding with benzo[h]quinoline

Authors

  • Ping-Chieh Hsieh,

    1. Department of Marine Environment and Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan
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  • Peter Brimblecombe,

    1. School of Environmental Sciences, University of East Anglia, Norwich, UK
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  • Chon-Lin Lee,

    Corresponding author
    1. Department of Marine Environment and Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan
    2. Kuroshio Research Group, Asia-Pacific Ocean Research Center, Kaohsiung, Taiwan
    3. Center for Emerging Contaminants Research, National Sun Yat-sen University, Kaohsiung, Taiwan
    • Department of Marine Environment and Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan
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  • Shih-Han Hsu

    1. Department of Marine Environment and Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan
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Abstract

The binding constants (KDOC) of the mixture of benzo[h]quinoline and its protonated analog, benzo[h]quinolinium, to four types of humic substances obtained from the International Humic Substances Society were determined by the fluorescence quenching method. A simple mixing model was used to eliminate the fluorescent interference from the minor analog in the solution and to deduce Kmix, which represents the overall binding as the sum of that for the individual analogs. The characteristics of humic substances, especially their hydrophobicity and aromaticity, established by principal component analysis of structural and elemental compositions, were the main determinants of the binding affinity with both benzo[h]quinoline and benzo[h]quinolinium (KBQ and equation image) across a range of pH values. The strongest overall affinity of benzo[h]quinoline for humic substances is observed near pH 4 and with more hydrophobic humic substances, which suggests possible choices in attempts at remediation of benzo[h]quinoline containing particles with humic substances. Environ. Toxicol. Chem. 2012;31:246–252. © 2011 SETAC

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