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Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning stress and growth

Authors

  • Andrea Ziková,

    Corresponding author
    1. Department of Fisheries and Hydrobiology, Mendel University of Agriculture and Forestry, Zemědělská 1, 613 00 Brno, Czech Republic
    2. Centre for Cyanobacteria and Their Toxins (Institute of Botany, Czech Academy of Sciences and RECETOX, Masaryk University), Kamenice 126/3, 625 00 Brno, Czech Republic
    • Department of Fisheries and Hydrobiology, Mendel University of Agriculture and Forestry, Zemědělská 1, 613 00 Brno, Czech Republic.
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  • Achim Trubiroha,

    1. Department of Aquaculture and Ecophysiology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany
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  • Claudia Wiegand,

    1. Department of Aquaculture and Ecophysiology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany
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  • Sven Wuertz,

    1. Laboratory of Environmental Toxicology (LETox), Centro Interdisciplinar de Investigaçao Marinha e Ambiental, Rua dos Bragas 289, 4050-123 Porto, Portugal
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  • Bernhard Rennert,

    1. Department of Aquaculture and Ecophysiology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany
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  • Stephan Pflugmacher,

    1. Department of Aquaculture and Ecophysiology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany
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  • Radovan Kopp,

    1. Department of Fisheries and Hydrobiology, Mendel University of Agriculture and Forestry, Zemědělská 1, 613 00 Brno, Czech Republic
    2. Centre for Cyanobacteria and Their Toxins (Institute of Botany, Czech Academy of Sciences and RECETOX, Masaryk University), Kamenice 126/3, 625 00 Brno, Czech Republic
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  • Jan Mareš,

    1. Department of Fisheries and Hydrobiology, Mendel University of Agriculture and Forestry, Zemědělská 1, 613 00 Brno, Czech Republic
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  • Werner Kloas

    1. Department of Aquaculture and Ecophysiology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany
    2. Department of Endocrinology, Institute of Biology, Humboldt University, 10178 Berlin, Germany
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  • Presented at the 1st International Workshop on Aquatic Toxicology and Biomonitoring, Vodnany, Czech Republic, August 27–29, 2008.

Abstract

Diets containing Microcystis with considerable amounts of the cyanotoxin microcystin-LR (MC-LR) were fed to determine their impact on the physiological performance of the omnivorous Nile tilapia (Oreochromis niloticus) with regard to stress and growth performance. Four different diets were prepared based on a commercial diet (control, MC-5% [containing 5% dried Microcystis biomass], MC-20% [containing 20% dried Microcystis biomass], and Arthrospira-20% [containing 20% dried Arthrospira sp. biomass without toxin]) and fed to female Nile tilapia. Blood and tissue samples were taken after 1, 7, and 28 d, and MC-LR was quantified in gills, muscle, and liver by using high-performance liquid chromatography (HPLC). Only in the liver were moderate concentrations of MC-LR detected. The stress hormone cortisol and glucose were analyzed from plasma, suggesting that all modified diets caused only minor to moderate stress, which was confirmed by analyses of hepatic glycogen. In addition, the effects of the different diets on growth performance were investigated by determining gene expression of hypophyseal growth hormone (GH) and hepatic insulin-like growth factor-I (IGF-I). For all diets, quantitative reverse transcription-polymerase chain reaction (RT-qPCR) demonstrated no significant effect on gene expression of the major endocrine hormones of the growth axis, whereas classical growth data, including growth and feed conversion ratio, displayed slight inhibitory effects of all modified diets independent of their MC-LR content. However, no significant change was found in condition or hepatosomatic index among the various diets, so it seems feasible that dried cyanobacterial biomass might be even used as a component in fish diet for Nile tilapia, which requires further research in more detail. Environ. Toxicol. Chem. 2010;29:561–568. © 2009 SETAC

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