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Effect of nitrite on early-life stages of common carp (Cyprinus carpio L.)

Authors

  • Hana Kroupova,

    Corresponding author
    1. Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic
    • Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic.
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  • Miroslav Prokes,

    1. Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, v.v.i., Kvetna 8, 603 65 Brno, Czech Republic
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  • Stanislava Macova,

    1. University of Veterinary and Pharmaceutical Sciences, Palackeho 1/3, 612 42 Brno, Czech Republic
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  • Milan Penaz,

    1. Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, v.v.i., Kvetna 8, 603 65 Brno, Czech Republic
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  • Vlastimil Barus,

    1. Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, v.v.i., Kvetna 8, 603 65 Brno, Czech Republic
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  • Ladislav Novotny,

    1. Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
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  • Jana Machova

    1. Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic
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  • Presented at the 1st International Workshop on Aquatic Toxicology and Biomonitoring, Vodnany, Czech Republic, August 27–29, 2008.

Abstract

A one-month chronic exposure of common carp larvae and embryos to nitrite revealed significant (p < 0.01) differences in total accumulated mortality in fish exposed to 33, 67, and 330 mg/L NOmath image compared with controls. At the highest concentration, all fish died within 8 d of exposure. On the basis of accumulated mortality in the experimental groups, lethal concentrations of nitrite were estimated at 29 d LC50 = 88 mg/L NOmath image; lowest-observed-effect concentration (LOEC) = 28 mg/L NOmath image; and no-observed-effect concentration (NOEC) = 7 mg/L NOmath image. Fulton's condition factor values were significantly lower in fish from all experimental groups compared with controls. By day 12, fish exposed to 33 and 67 mg/L NOmath image had significantly lower mass and total length compared with controls. No significant negative effects of nitrite at the concentrations tested (0.7–330 mg/L NOmath image, at 10 mg/L Cl) on hatching or embryo viability were demonstrated, but significant differences in early ontogeny among groups were noted. Fish from all the concentrations showed a dose-related delay in development compared with the controls. Lordosis, kyphosis, scoliosis, and body shortening were observed at all concentrations and in controls, as was yolk sac deformation and edema, eye deformation, and cardiac edema. The incidence of these malformations was positively correlated with nitrite concentration. Histopathology revealed epidermal spongiosis; edema and hyperplasia of the gill epithelium, including hypertrophy and hyperplasia of eosinophilic granular cells (chloride cells); and interstitial edema of skeletal muscle in fish exposed to 67 mg/L NOmath image. Similar, but milder, changes were observed at lower nitrite concentrations. Environ. Toxicol. Chem. 2010;29:535–540. © 2009 SETAC

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