First International Workshop on Aquatic Toxicology and Biomonitoring

Toxicity of cyanobacterial bloom in the eutrophic dam reservoir (Southeast Poland)†

  1. Anna Sierosławska1,*,
  2. Anna Rymuszka1,
  3. Renata Kalinowska2,
  4. Tadeusz Skowroński1,2,
  5. Adam Bownik1,
  6. Barbara Pawlik-Skowrońska2

Article first published online: 10 DEC 2009

DOI: 10.1002/etc.86

Issue

Environmental Toxicology and Chemistry

Environmental Toxicology and Chemistry

Volume 29, Issue 3, pages 556–560, March 2010

Additional Information

How to Cite

Sierosławska, A., Rymuszka, A., Kalinowska, R., Skowroński, T., Bownik, A. and Pawlik-Skowrońska, B. (2010), Toxicity of cyanobacterial bloom in the eutrophic dam reservoir (Southeast Poland). Environmental Toxicology and Chemistry, 29: 556–560. doi: 10.1002/etc.86

Author Information

  1. 1

    The John Paul II Catholic University of Lublin, Department of Physiology and Ecotoxicology, Norwida 4, 20-061 Lublin, Poland

  2. 2

    Centre for Ecological Research, Polish Academy of Sciences, Experimental Station, Niecała 18, 20-080 Lublin, Poland

*The John Paul II Catholic University of Lublin, Department of Physiology and Ecotoxicology, Norwida 4, 20-061 Lublin, Poland.

  1. †

    Presented at the First International Workshop on Aquatic Toxicology and Biomonitoring, Vodnany, Czech Republic, August 27–29, 2008.

Publication History

  1. Issue published online: 17 FEB 2010
  2. Article first published online: 10 DEC 2009
  3. Accepted manuscript online: 10 DEC 2009 12:00AM EST
  4. Manuscript Accepted: 22 APR 2009
  5. Manuscript Revised: 6 JAN 2009
  6. Manuscript Received: 2 OCT 2008

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (98K)

Keywords:

  • Cyanobacterial bloom;
  • Anatoxin-a;
  • Daphnia pulex;
  • Tetrahymena thermophila

Abstract

Cyanobacterial bloom was observed in a highly eutrophic dam reservoir, Zemborzycki, near Lublin (SE Poland) over a warm period in the year 2007. The water bloom consisted of several cyanobacterial taxa: Anabaena circinalis, Anabaena spiroides, Anabaena flos-aquae, Planktothrix agardhii, Aphanizomenon flos-aquae, Aphanizomenon gracile, and Microcystis flos-aquae. Anabaena spp., and Aphanizomenon spp., potential producers of neurotoxic anatoxin-a, quantitatively predominated in the studied bloom. High-performance liquid chromatography (HPLC) analysis of surface scum sampled during Anabaena circinalis domination revealed the presence of anatoxin-a at a high concentration (1,035.59 µg per liter of surface scum). At the same time, neither gas chromatography/mass spectrometry (GC/MS) nor microcystin enzyme-linked immunosorbent assay (ELISA) test showed the presence of other frequently found cyanotoxins, microcystins. Toxicity of cyanobacterial bloom was assessed by the crustacean acute toxicity test Daphtoxkit F™ pulex using Daphnia pulex, and by the chronic toxicity test Protoxkit F™ with a ciliate protozoan Tetrahymena thermophila. The crude extract of cyanobacterial scum showed high toxicity for Daphnia pulex, with 24-h median effective concentration (EC50) value of 90.3 µg/L of anatoxin-a, which corresponded to the cyanobacterial density in the scum of 1.01 g dry weight/L. For Tetrahymena thermophila, 24-h EC50 was lower, evaluated to be 60.48 µg/L of anatoxin-a, which corresponded to a cyanobacterial density of 0.68 g dry weight/L of the scum. On the basis of evaluated toxicity units, the cyanobacterial extract was classified at class IV toxicity, which means high toxic hazard. Environ. Toxicol. Chem. 2010;29:556–560. © 2009 SETAC

View Full Article (HTML) Get PDF (98K)