Determination of thiol compounds by automated headspace solid-phase microextraction with in-fiber derivatization
Article first published online: 5 APR 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Flavour and Fragrance Journal
Volume 21, Issue 3, pages 385–394, May/June 2006
How to Cite
Wang, Q., Chong, J. M. and Pawliszyn, J. (2006), Determination of thiol compounds by automated headspace solid-phase microextraction with in-fiber derivatization. Flavour Fragr. J., 21: 385–394. doi: 10.1002/ffj.1724
- Issue published online: 24 APR 2006
- Article first published online: 5 APR 2006
- Manuscript Accepted: 28 NOV 2005
- Manuscript Revised: 10 NOV 2005
- Manuscript Received: 10 SEP 2005
- in-fiber derivatization;
- solid-phase microextraction;
In this article, headspace solid-phase microextraction (HS-SPME) analysis of thiol compounds with both solid and aqueous samples was investigated using N-phenylmaleimide as an in-fiber derivatization reagent. The derivatives were analyzed using GC-MS. The HS-SPME method was executed on an automatic sampler and optimized by studying a variety of factors such as the selection of the fiber, extraction time, temperature, agitation speed, etc. Method validation was carried out by spiking known amounts of thiol compounds into the sample and calculating the concentration by standard addition. The relative standard deviations (RSD) for the majority of the thiols were less than 10%. The developed method resulted in a limit of detection (LOD) in the low microgram per liter for most of the thiols analyzed, which was about one order of magnitude lower than the HS-SPME without derivatization. Finally, the overall method was successfully applied for the determination of thiols in yellow onion, green onion and garlic. Copyright © 2006 John Wiley & Sons, Ltd.