Chromatographic separation of flaxseed phenolics

Authors

  • Dr. R. Amarowicz M. Sc,

    Corresponding author
    1. Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada
    2. Department of Food Sciences, Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, Olsztyn, Poland
    • Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, Canada
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  • P. K. J. P. D. Wanasundara,

    Corresponding author
    1. Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada
    • Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, Canada
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  • Prof. Dr. F. Shahidi

    Corresponding author
    1. Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada
    • Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, Canada
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Abstract

A column chromatography method for separation of phenolic compounds from flaxseed using Sephadex LH-20, RP-8 and silica gel is described. TLC analysis proved that separated fractions contained numerous phenolic compounds. All fractions exhibited a maximum UV absorption between 270 and 290 nm. Additional shoulders were observed between 274 and 350 nm. These results imply that lignans and their derivatives are the main phenolic compounds present in flaxseed.

Abstract

Chromatographische Trennung von Flachssamen-Phenolen

Es wird eine säulenchromatographische Methode zur Trennung von phenolischen Verbindungen von Flachssamen unter Verwendung von Sephadex LH-20, RP-8 und Silicagel beschrieben. TLC-Analysen zeigen, daß die getrennten Fraktionen zahlreiche phenolische Verbindungen enthalten. Alle Fraktionen weisen ein Maximum der UV-Absorption zwischen 270 und 290 nm auf, zusätzliche Schultern werden zwischen 274 und 350 nm beobachtet. Die Ergebnisse lassen darauf schließen, daß Lignane und ihre Derivate die wesentlichen Phenolverbindungen im Flachssamen sind.

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