Sulfated glycosaminoglycans from crown-of-thorns Acanthaster planci – extraction and quantification analysis
Article first published online: 14 DEC 2012
© 2012 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Food Science & Nutrition
Volume 1, Issue 1, pages 83–89, January 2013
How to Cite
Bahrom, N. A., Sirajudeen, K. N. S., Yip, G. W., Latiff, A. A. and Ghazali, F. C. (2013), Sulfated glycosaminoglycans from crown-of-thorns Acanthaster planci – extraction and quantification analysis. Food Science & Nutrition, 1: 83–89. doi: 10.1002/fsn3.10
- Issue published online: 8 JAN 2013
- Article first published online: 14 DEC 2012
- Manuscript Accepted: 25 OCT 2012
- Manuscript Revised: 14 OCT 2012
- Manuscript Received: 3 SEP 2012
- Agro-Biotechnology Institute Grant. Grant Number: 203/PPSK/6171139
- Fundamental Grant Research Scheme. Grant Numbers: 304/PPSK/6150088/T114, 203/PPSK/6171139
- A canthaster planci ;
- starfish and GAGs;
- sulfated glycosaminoglycans
In this article, the novel inventive steps for the extraction and quantification of sulfated glycosaminoglycan (GAG) from Acanthaster planci starfish, generally known as crown-of-thorns (COT), are reported. Starfish have been implicated with collagenous distributions within their body anatomy, thus making it a prima facie fact searching for the possibility that GAGs can be isolated from COT. In this study, total-, N-, and O-sulfated GAGs were extracted from three anatomical regions of the COT (integument, internal tissue, and coelomic fluid) and comparison was made. The result showed that body region of COT seemed to contain higher amount of sulfated GAGs as opposed to the arm region (55.79 ± 0.65 μg/mg was the highest amount in the body extracted from its coelomic fluid and 32.28 ± 3.14 μg/mg was the highest amount in the arm extracted from its internal tissue). COT's integument and coelomic fluid from its body region possessed the highest total of sulfated GAGs content with no significant difference (P < 0.05) between the two. All GAGs from COT comprised a higher percentage of N-sulfated GAGs than its counterpart, the O-sulfated GAGs. When compared with a similar previous study that used sea cucumbers as the sulfated GAGs source, COT possessed more total sulfated GAGs content per milligram as compared with the sea cucumber generally. This result seems to unveil this marine species' advantage per se pertaining to GAGs extraction biomass applicability. Thus, COT could now be the better alternative source for production technology of total-, N-, and O-sulfated GAGs.