Antioxidant activities of chick embryo egg hydrolysates
Article first published online: 18 DEC 2013
© 2013 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Food Science & Nutrition
Volume 2, Issue 1, pages 58–64, January 2014
How to Cite
Food Science & Nutrition 2014; 2(1): 58–64
- Issue published online: 13 JAN 2014
- Article first published online: 18 DEC 2013
- Manuscript Revised: 10 OCT 2013
- Manuscript Accepted: 10 OCT 2013
- Manuscript Received: 9 JUL 2013
- National Natural Science Foundation of China. Grant Numbers: 307607, 30365&31301426
- Natural Science Foundation of Hubei Province of China. Grant Number: 2013CFB198
- Antioxidant activities;
- chick embryo egg hydrolysates;
- nutritive value
Chick embryo egg hydrolysates (CEEH) were obtained by enzymatic hydrolysis of chick embryo egg in vitro-simulated gastrointestinal digestion. The antioxidant activities of CEEH were investigated by employing three in vitro assays, including the 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate)/1,1-diphenyl-2-picrylhydrazyl (ABTS/DPPH)/hydroxyl radical-scavenging assays. The radical-scavenging effect of CEEH (1.0 mg/mL) was in a dose-dependent manner, with the highest trolox equivalent antioxidant capacity for ABTS, DPPH, and that of hydroxyl radicals found to be 569, 2097, and 259.6 μmol/L, respectively; whereas the trolox equivalent antioxidant capacity of unhatched egg for ABTS, DPPH, and that of hydroxyl radicals were found to be 199, 993, and 226.5 μmol/L, respectively. CEEH showed stronger scavenging activity than the hydrolysates of unhatched egg against free radicals such as ABTS, DPPH, and hydroxyl radicals. The antioxidant amino acid analysis indicated that the 14-day CEEH possess more antioxidant amino acids than that of the unhatched egg. In addition, essential amino acids analysis showed that the 14-day CEEH have the highest nutritional value. Combined with the results of the amino acid profiles, CEEH were believed to have higher nutritive value in addition to antioxidant activities than the unhatched egg.