Original Research Paper

Membrane-Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) Wired via Specific Os-Redox Polymers

  1. L. Stoica1,
  2. N. Dimcheva1,
  3. Y. Ackermann1,
  4. K. Karnicka2,
  5. D. A. Guschin1,
  6. P. J. Kulesza2,
  7. J. Rogalski3,
  8. D. Haltrich4,
  9. R. Ludwig4,
  10. L. Gorton5,
  11. W. Schuhmann1

Article first published online: 11 FEB 2009

DOI: 10.1002/fuce.200800033

Issue

Fuel Cells

Fuel Cells

Special Issue: “Biofuel Cells”

Volume 9, Issue 1, pages 53–62, February, 2009

Additional Information

How to Cite

Stoica, L., Dimcheva, N., Ackermann, Y., Karnicka, K., Guschin, D. A., Kulesza, P. J., Rogalski, J., Haltrich, D., Ludwig, R., Gorton, L. and Schuhmann, W. (2009), Membrane-Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) Wired via Specific Os-Redox Polymers. Fuel Cells, 9: 53–62. doi: 10.1002/fuce.200800033

Author Information

  1. 1

    Analytische Chemie–Elektoanalytik und Sensorik, Ruhr-Universität Bochum, Universitätstr. 150, D-44780 Bochum, Germany

  2. 2

    Department of Chemistry, University of Warsaw, Pasteura 1, PL-02-093 Warsaw, Poland

  3. 3

    Department of Biochemistry, Maria Curie Sklodowska University, Lublin, Poland

  4. 4

    Department of Food Sciences and Technology, Division of Food Biotechnology, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, A-1190 Vienna, Austria

  5. 5

    Department of Analytical Chemistry, Lund University, P.O. Box 124, 226 46 Lund, Sweden

Publication History

  1. Issue published online: 11 FEB 2009
  2. Article first published online: 11 FEB 2009
  3. Manuscript Accepted: 15 OCT 2008
  4. Manuscript Received: 1 MAY 2008

Funded by

  • MIWFT-NRW (Düsseldorf)
  • The Swedish Research Council
  • Austrian Science Fund. Grant Number: FWF L395-B11

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Keywords:

  • Biofuel Cell;
  • Cellobiose;
  • Cellobiose Dehydrogenase;
  • Laccase;
  • Lactose;
  • Os-Redox Polymer;
  • Oxygen Reduction Reaction

Abstract

A membrane-free biofuel cell (BFC) is reported based on enzymes wired to graphite electrodes by means of Os-complex modified redox polymers. For the anode cellobiose dehydrogenase (CDH) is used as a biocatalyst whereas for the cathode a laccase was applied. This laccase is a high-potential laccase and hence able to reduce O2 to H2O at a formal potential higher than +500 mV versus Ag/AgCl. In order to establish efficient electrochemical contact between the enzymes and graphite electrodes electrodeposition polymers containing Os-complex with specifically designed monomer compositions and formal potentials of the coordinatively bound Os-complex were synthesised and used to wire the enzymes to the electrodes. The newly designed CDH/Os-redox polymer anode was characterised at different pH values and optimised with respect to the nature of the polymer and the enzyme-to-polymer ratio. The resulting BFC was evaluated running on β-lactose as a fuel and air/O2 as an oxidising agent. The power output, the maximum current density and the electromotor force (Eemf) were found to be affected by the pH value, resulting in a maximum power output of 1.9 μW cm–2 reached at pH 4.3, a maximum current density of about 13 μA cm–2 at pH 3.5, and the highest Eemf approaching 600 mV at pH 4.0.

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